beta actin Polyclonal Antibody-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（103）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Sequence-dependent effects of hematoporphyrin derivatives (HPD) photodynamic therapy and cisplatin on lung adenocarcinoma cells

IF: 3.100

Journal： Photodiagnosis and Photodynamic Therapy (2024)

DOI：10.1016/j.pdpdt.2024.104102

Reactivity：Human
D-alanine suppressed osteoclastogenesis derived from bone marrow macrophages and downregulated ERK/p38 signalling pathways

IF: 3.000

Journal： ARCHIVES OF ORAL BIOLOGY (2024)

DOI：10.1016/j.archoralbio.2024.105912

Sample Type：bone marrow-derived macrophages (BMMs)
Phycocyanin attenuates skeletal muscle damage and fatigue via modulation of Nrf2 and IRS-1/AKT/mTOR pathway in exercise-induced oxidative stress in rats

IF: 2.900

Journal： PLoS One (2024)

DOI：10.1371/journal.pone.0310138

Reactivity：Rat

Sample Type：quadriceps muscle tissue,skeletal muscle tissue
Effect of baicalin and baicalin-bovine serum albumin nanoparticle against bendiocarb exposure in rats

IF: 2.200

Journal： Toxicology Research (2024)

DOI：10.1093/toxres/tfae134

Reactivity：Rat

Sample Type：kidney tissue,liver tissue
Analysis of the Anticancer Mechanism of OR3 Pigment from Streptomyces coelicolor JUACT03 Against the Human Hepatoma Cell Line Using a Proteomic Approach

IF: 1.800

Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2024)

DOI：10.1007/s12013-024-01258-0

Reactivity：Human
Hyperactive neuronal autophagy depletes BDNF and impairs adult hippocampal neurogenesis in a corticosterone-induced mouse model of depression

IF: 12.400

Journal： Theranostics (2023)

DOI：10.7150/thno.81067

PMID：36793868

Reactivity：Mouse

Sample Type：Hippocampal dentate gyrus (DG)
Investigation of the efficacy of diosmin against organ damage caused by bendiocarb in male Wistar albino rats

IF: 5.800

Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)

DOI：10.1007/s11356-023-26105-2

PMID：36905537

Reactivity：Rat

Sample Type：hepatic tissue
Dimethyl fumarate ameliorates fungal keratitis by limiting fungal growth and inhibiting pyroptosis

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.109721

PMID：36641891

Reactivity：Human,Mouse

Sample Type：corneal tissue
Honokiol reduces fungal burden and ameliorate inflammation lesions of Aspergillus fumigatus keratitis via Dectin-2 down-regulation

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.109849

PMID：36933490

Reactivity：Mouse

Sample Type：corneas
A combination of metformin and epigallocatechin gallate potentiates glioma chemotherapy in vivo

IF: 5.600

Journal： Frontiers in Pharmacology (2023)

DOI：10.3389/fphar.2023.1096614

PMID：37025487

Reactivity：Rat

Sample Type：C6 cell-Xenograft

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E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays