beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Anti-Inflammatory Efficacy of Nanobody Specific to β-Glucan on a Fungal Cell Wall in a Murine Model of Fungal Keratitis

IF: 4.0

Journal： ACS Infectious Diseases (2024)

DOI：10.1021/acsinfecdis.4c00334

Reactivity：Mouse

Sample Type：cornea tissue
FDI-6 inhibits VEGF-B expression in metastatic breast cancer: a combined in vitro and in silico study

IF: 3.9

Journal： MOLECULAR DIVERSITY (2024)

DOI：10.1007/s11030-024-10891-z

Reactivity：Human
Downregulation of IL-1β/p38 mitogen activated protein kinase pathway by diacerein protects against kidney ischemia/reperfusion injury in rats

IF: 3.8

Journal： CYTOKINE (2024)

DOI：10.1016/j.cyto.2024.156511

Reactivity：1:2000
Passiflora edulis extract ameliorates HFD-induced hepatic steatosis mediated through Nrf2 and IRS-1 activation, NFκB suppression, and hepatic lipid metabolism and bile acid modulation in obese rats

IF: 3.8

Journal： Journal of Functional Foods (2024)

DOI：10.1016/j.jff.2024.106351

Reactivity：Rat

Sample Type：liver tissue
SLC45A4 is involved in malignant progression of ovarian cancer through glycolytic metabolic reprogramming

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-74249-z

Reactivity：Human
Hormetic effects of a cannabinoid system component, 2-arachidonoyl glycerol, on cell viability and expression profile of growth factors in cultured mouse Sertoli cells: Friend or foe of male fertility?

IF: 3.3

Journal： REPRODUCTIVE TOXICOLOGY (2024)

DOI：10.1016/j.reprotox.2024.108575

PMID：38462211

Reactivity：Mouse
Sequence-dependent effects of hematoporphyrin derivatives (HPD) photodynamic therapy and cisplatin on lung adenocarcinoma cells

IF: 3.1

Journal： Photodiagnosis and Photodynamic Therapy (2024)

DOI：10.1016/j.pdpdt.2024.104102

Reactivity：Human
D-alanine suppressed osteoclastogenesis derived from bone marrow macrophages and downregulated ERK/p38 signalling pathways

IF: 3.0

Journal： ARCHIVES OF ORAL BIOLOGY (2024)

DOI：10.1016/j.archoralbio.2024.105912

Sample Type：bone marrow-derived macrophages (BMMs)
Phycocyanin attenuates skeletal muscle damage and fatigue via modulation of Nrf2 and IRS-1/AKT/mTOR pathway in exercise-induced oxidative stress in rats

IF: 2.9

Journal： PLoS One (2024)

DOI：10.1371/journal.pone.0310138

Reactivity：Rat

Sample Type：quadriceps muscle tissue,skeletal muscle tissue
Effect of baicalin and baicalin-bovine serum albumin nanoparticle against bendiocarb exposure in rats

IF: 2.2

Journal： Toxicology Research (2024)

DOI：10.1093/toxres/tfae134

Reactivity：Rat

Sample Type：kidney tissue,liver tissue

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