beta actin Polyclonal Antibody-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（103）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Candidalysin amplifies the immune inflammatory response in Candida albicans keratitis through the TREM-1/DAP12 pathway

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.110195

PMID：37087869

Reactivity：Mouse,Candida albicans strain 2.4159

Sample Type：corneal tissues
Vitamin D Improves Cognitive Impairment and Alleviates Ferroptosis via the Nrf2 Signaling Pathway in Aging Mice

IF: 5.600

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

DOI：10.3390/ijms242015315

PMID：37894993

Reactivity：Mouse

Sample Type：hippocampus
Pseudolaric Acid B Ameliorates Fungal Keratitis Progression by Suppressing Inflammation and Reducing Fungal Load

IF: 5.300

Journal： ACS Infectious Diseases (2023)

DOI：10.1021/acsinfecdis.2c00536

PMID：37141176

Reactivity：Mouse

Sample Type：cornea
Palmitic Acid Inhibits the Growth and Metastasis of Gastric Cancer by Blocking the STAT3 Signaling Pathway

IF: 5.200

Journal： Cancers (2023)

DOI：10.3390/cancers15020388

PMID：36672337

Reactivity：Human

Sample Type：gastric cancer tissue
Benzyl isothiocyanate improves the prognosis of Aspergillus fumigatus keratitis by reducing fungal load and inhibiting Mincle signal pathway

IF: 5.200

Journal： Frontiers in Microbiology (2023)

DOI：10.3389/fmicb.2023.1119568

PMID：36876115

Reactivity：Mouse

Sample Type：corneas
Reduced Cerebellar BDNF Availability Affects Postnatal Differentiation and Maturation of Granule Cells in a Mouse Model of Cholesterol Dyshomeostasis

IF: 5.100

Journal： MOLECULAR NEUROBIOLOGY (2023)

DOI：10.1007/s12035-023-03435-3

PMID：37314654

Reactivity：Mouse

Sample Type：cerebella
Probiotics as an Alternative to Antibiotics: Genomic and Physiological Characterization of Aerobic Spore Formers from the Human Intestine

IF: 4.500

Journal： Microorganisms (2023)

DOI：10.3390/microorganisms11081978

PMID：37630538

Reactivity：Human
Vitamin D may assist the UPR against sodium fluoride-induced damage by reducing RIPK1, ATG5, BECN1, oxidative stress and increasing Caspase-3 in the osteoblast MC3T3-E1 cell line

IF: 3.500

Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2023)

DOI：10.1016/j.jtemb.2023.127293

PMID：37677921

Reactivity：Mouse
Inhibition of AIF-1 alleviates laser-induced macular neovascularization by inhibiting endothelial cell proliferation via restrained p44/42 MAPK signaling pathway

IF: 3.400

Journal： EXPERIMENTAL EYE RESEARCH (2023)

DOI：10.1016/j.exer.2023.109474

PMID：37080383

Reactivity：Mouse

Sample Type：RPE-choroid-sclera complexes
Different Proteins Regulated Apoptosis, Proliferation and Metastasis of Lung Adenocarcinoma After Radiotherapy at Different Time

IF: 3.300

Journal： Cancer Management and Research (2023)

DOI：10.2147/CMAR.S219967

PMID：32308480

Reactivity：Human

Sample Type：Cell lysate

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays