beta actin Polyclonal Antibody-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（103）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Chuanzhitongluo inhibits neuronal apoptosis in mice with acute ischemic stroke by regulating the PI3K/AKT signaling pathway

IF: 3.300

Journal： NEUROSCIENCE (2023)

DOI：10.1016/j.neuroscience.2023.11.011

PMID：38040086

Reactivity：Mouse

Sample Type：brain tissue
Achyranthes bidentate extracts protect the IL-1β-induced osteoarthritis of SW1353 chondrocytes

IF: 2.800

Journal： JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2023)

DOI：10.1016/j.jbiosc.2023.09.008

PMID：37778956

Reactivity：Human
The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study

IF: 2.700

Journal： Journal of Pain Research (2023)

DOI：10.2147/JPR.S400871

PMID：36815126

Reactivity：Rat

Sample Type：dorsal root ganglion (DRG) tissue, intervertebral disc tissue
Antiobesity Effect of Lacticaseibacillus paracasei LM-141 on High-Fat Diet-Induced Rats through Alleviation of Inflammation and Insulin Resistance

IF: 2.650

Journal： Evidence-based Complementary and Alternative Medicine (2023)

DOI：10.1155/2023/1011591

PMID：37114144

Reactivity：Rat

Sample Type：hepatic tissues
Glabridin Inhibits Aspergillus fumigatus Growth and Alleviate Inflammation Mediated by Dectin-2 and NLRP3 Inflammasome

IF: 2.000

Journal： CURRENT EYE RESEARCH (2023)

DOI：10.1080/02713683.2022.2164779

PMID：36785524

Reactivity：Mouse
Role of Inhibiting Inflammation of LC3-Associated Phagocytosis in Dry Eye Disease

IF: 2.000

Journal： CURRENT EYE RESEARCH (2023)

DOI：10.1080/02713683.2023.2262169

PMID：37732765

Reactivity：Mouse

Sample Type：corneas
Oxidized chondroitin sulfate eye drops ameliorate the prognosis of fungal keratitis with anti-inflammatory and antifungal effects

IF: 7.571

Journal： Journal of Materials Chemistry B (2022)

DOI：10.1039/D2TB00114D

PMID：36070420

Reactivity：Mouse

Sample Type：corneas
OAT3 mediates methotrexate resistance in the treatment of rheumatoid arthritis

IF: 7.419

Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)

DOI：10.1016/j.biopha.2022.113558

PMID：36076621

Reactivity：Rat

Sample Type：synovium
Femtosecond laser attenuates oxidative stress, inflammation, and liver fibrosis in rats: Possible role of PPARγ and Nrf2/HO-1 signaling

IF: 6.780

Journal： LIFE SCIENCES (2022)

DOI：10.1016/j.lfs.2022.120877

PMID：35963297

Reactivity：Rat

Sample Type：liver
The molecular mechanism of baicalein repressing progression of gastric cancer mediating miR-7/FAK/AKT signaling pathway

IF: 6.656

Journal： PHYTOMEDICINE (2022)

DOI：10.1016/j.phymed.2022.154046

PMID：35306368

Reactivity：Human

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E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays