beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Analysis of the Anticancer Mechanism of OR3 Pigment from Streptomyces coelicolor JUACT03 Against the Human Hepatoma Cell Line Using a Proteomic Approach

IF: 1.8

Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2024)

DOI：10.1007/s12013-024-01258-0

Reactivity：Human
Hyperactive neuronal autophagy depletes BDNF and impairs adult hippocampal neurogenesis in a corticosterone-induced mouse model of depression

IF: 12.400

Journal： Theranostics (2023)

DOI：10.7150/thno.81067

PMID：36793868

Reactivity：Mouse

Sample Type：Hippocampal dentate gyrus (DG)
Investigation of the efficacy of diosmin against organ damage caused by bendiocarb in male Wistar albino rats

IF: 5.800

Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)

DOI：10.1007/s11356-023-26105-2

PMID：36905537

Reactivity：Rat

Sample Type：hepatic tissue
Dimethyl fumarate ameliorates fungal keratitis by limiting fungal growth and inhibiting pyroptosis

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.109721

PMID：36641891

Reactivity：Human,Mouse

Sample Type：corneal tissue
Honokiol reduces fungal burden and ameliorate inflammation lesions of Aspergillus fumigatus keratitis via Dectin-2 down-regulation

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.109849

PMID：36933490

Reactivity：Mouse

Sample Type：corneas
A combination of metformin and epigallocatechin gallate potentiates glioma chemotherapy in vivo

IF: 5.600

Journal： Frontiers in Pharmacology (2023)

DOI：10.3389/fphar.2023.1096614

PMID：37025487

Reactivity：Rat

Sample Type：C6 cell-Xenograft
Candidalysin amplifies the immune inflammatory response in Candida albicans keratitis through the TREM-1/DAP12 pathway

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.110195

PMID：37087869

Reactivity：Mouse,Candida albicans strain 2.4159

Sample Type：corneal tissues
Vitamin D Improves Cognitive Impairment and Alleviates Ferroptosis via the Nrf2 Signaling Pathway in Aging Mice

IF: 5.6

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

DOI：10.3390/ijms242015315

PMID：37894993

Reactivity：Mouse

Sample Type：hippocampus
Pseudolaric Acid B Ameliorates Fungal Keratitis Progression by Suppressing Inflammation and Reducing Fungal Load

IF: 5.300

Journal： ACS Infectious Diseases (2023)

DOI：10.1021/acsinfecdis.2c00536

PMID：37141176

Reactivity：Mouse

Sample Type：cornea
Palmitic Acid Inhibits the Growth and Metastasis of Gastric Cancer by Blocking the STAT3 Signaling Pathway

IF: 5.200

Journal： Cancers (2023)

DOI：10.3390/cancers15020388

PMID：36672337

Reactivity：Human

Sample Type：gastric cancer tissue

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