beta actin Polyclonal Antibody（E-AB-40338）|-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

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货号：E-AB-40338
文献（119）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Eugenol protects against Aspergillus fumigatus keratitis by inhibiting inflammatory response and reducing fungal load

IF: 5.195

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2022)

DOI：10.1016/j.ejphar.2022.174955

PMID：35436473

Reactivity：Mouse,Human

Sample Type：corneas
Hibiscus sabdariffa extract improves hepatic steatosis, partially through IRS-1/Akt and Nrf2 signaling pathways in rats fed a high fat diet

IF: 4.996

Journal： Scientific Reports (2022)

DOI：10.1038/s41598-022-11027-9

PMID：35487948

Reactivity：Rat

Sample Type：liver tissue
Paeonol protects against acute pancreatitis by Nrf2 and NF-κB pathways in mice

IF: 4.810

Journal： JOURNAL OF PHARMACY AND PHARMACOLOGY (2022)

DOI：10.1093/jpp/rgac065

PMID：36170125

Reactivity：Mouse

Sample Type：pancreatic tissue
The therapeutic potential of chondroitin sulfate in Aspergillus fumigatus keratitis

IF: 4.174

Journal： MOLECULAR IMMUNOLOGY (2022)

DOI：10.1016/j.molimm.2022.04.007

PMID：35504058

Reactivity：Mouse

Sample Type：corneas
Streptococcus pneumoniae exerts oxidative stress, subverts antioxidant signaling and autophagy in human corneal epithelial cells that is alleviated by tert-Butylhydroquinone

IF: 4.148

Journal： MEDICAL MICROBIOLOGY AND IMMUNOLOGY (2022)

DOI：10.1007/s00430-022-00731-y

PMID：35325292

Reactivity：Human
Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells

IF: 3.946

Journal： JOURNAL OF PERIODONTAL RESEARCH (2022)

DOI：10.1111/jre.12973

PMID：35037251

Reactivity：Human
Hypermethylation of the GRHL2 promoter region is associated with ovarian endometriosis

IF: 3.923

Journal： REPRODUCTION (2022)

DOI：10.1530/REP-21-0383

Reactivity：Human
Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke

IF: 3.715

Journal： BRAIN RESEARCH BULLETIN (2022)

DOI：10.1016/j.brainresbull.2022.09.015

PMID：36152772

Reactivity：Mouse

Sample Type：brain tissue
Thymol Ameliorates Aspergillus fumigatus Keratitis by Downregulating the TLR4/ MyD88/ NF-kB/ IL-1β Signal Expression and Reducing Necroptosis and Pyroptosis

IF: 3.277

Journal： JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2022)

DOI：10.4014/jmb.2207.07017

Reactivity：Mouse

Sample Type：cornea
Down-regulation of miR-18b-5p protects against splenic hemorrhagic shock by directly targeting HIF-1α/iNOS pathway

IF: 3.152

Journal： IMMUNOBIOLOGY (2022)

DOI：10.1016/j.imbio.2022.152188

PMID：35180673

Reactivity：Rat

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