beta actin Polyclonal Antibody-Elabscience伊莱瑞特【官网】

beta actin Polyclonal Antibody

一键复制产品信息

货号：E-AB-40338
文献（103）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, RAW264.7, Rat brain, Mouse brain Verified Samples in IHC: Human colon, Human breast cancer, Mouse heart, Rat heart
Dilution	WB 1:3000-1:30000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Actin, cytoplasmic 2 protein
Abbre	ACTBB
Synonyms	A26C1A, Actin beta, Actin cytoplasmic 1, Actx, b actin, ACTB
Swissprot	Q7ZVF9
Calculated MW	41 kDa
Observed MW	41 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Isotype, Tags and Cell markers, Signal Transduction
Conjugation	Unconjugated
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Western Blot analysis of A431 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat brain and Mouse brain using beta actin Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Raw264.7 cells using beta actin Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Mouse heart using beta actin Polyclonal Antibody at dilution of 1:200

Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polyclonal Antibody at dilution of 1:200

Revealing the suppressive role of protein kinase C delta and p38 mitogen-activated protein kinase (MAPK)/NF-κB axis associates with lenvatinib-inhibited progression in hepatocellular carcinoma in vitro and in vivo

IF: 6.530

Journal： BIOMEDICINE & PHARMACOTHERAPY (2021)

DOI：10.1016/j.biopha.2021.112437

PMID：34864311

Reactivity：Human
LncRNA MALAT1 Facilitates Ovarian Cancer Progression through Promoting Chemoresistance and Invasiveness in the Tumor Microenvironment

IF: 5.924

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

DOI：10.3390/ijms221910201

PMID：34638541

Reactivity：Human
Eleutheroside B-loaded poly (lactic-co-glycolic acid) nanoparticles protect against renal fibrosis via Smad3-dependent mechanism

IF: 5.882

Journal： PHYTOTHERAPY RESEARCH (2021)

DOI：10.1002/ptr.7293

PMID：34585457

Reactivity：Human,Mouse

Sample Type：kidney
Neuroprotective effect of pseudoginsenoside-F11 on permanent cerebral ischemia in rats by regulating calpain activity and NR2A submit-mediated AKT-CREB signaling pathways

IF: 5.340

Journal： PHYTOMEDICINE (2021)

DOI：10.1016/j.phymed.2021.153847

PMID：34836744

Reactivity：Rat

Sample Type：brain
Galectin-3 plays an important pro-inflammatory role in A. fumigatus keratitis by recruiting neutrophils and activating p38 in neutrophils

IF: 4.932

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)

DOI：10.1016/j.intimp.2021.107706

PMID：33933850

Reactivity：Mouse

Sample Type：corneas and
IL-36α Exerts Proinflammatory Effects in Aspergillus fumigatus Keratitis of Mice Through the Pathway of IL-36α/IL-36R/NF-κB

IF: 4.799

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2021)

DOI：10.1167/iovs.62.4.16

PMID：33851975

Reactivity：Mouse

Sample Type：Cell sample
Baicalein Inhibits Gastric Cancer Cell Proliferation and Migration through a FAK Interaction via AKT/mTOR Signaling

IF: 4.667

Journal： AMERICAN JOURNAL OF CHINESE MEDICINE (2021)

DOI：10.1142/S0192415X21500245

PMID：33641654

Reactivity：Human
4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis

IF: 4.104

Journal： PROSTATE (2021)

DOI：10.1002/pros.24205

PMID：34320251

Reactivity：Mouse

Sample Type：prostate
Lenvatinib Inhibits AKT/NF-κB Signaling and Induces Apoptosis Through Extrinsic/Intrinsic Pathways in Non-small Cell Lung Cancer

IF: 2.480

Journal： ANTICANCER RESEARCH (2021)

DOI：10.21873/anticanres.14757

Reactivity：Human
α- MSH Plays Anti-inflammatory and Anti-fungal Role in Aspergillus Fumigatus Keratitis

IF: 2.424

Journal： CURRENT EYE RESEARCH (2021)

DOI：10.1080/02713683.2021.2006235

PMID：34766863

Reactivity：Mouse

Sample Type：corneas

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays