beta Tubulin Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: Human;Mouse;E.coli;Scallop;Zebrafish
- 应用性: WB;IF
Verified Samples |
Verified Samples in WB: Hela, Caco-2, HT-29, NIH/3T3, Raw264.7, Rat liver, HEK293 |
Dilution | WB 1:5000-10000, IF 1:2000-4000 |
Clonality | Monoclonal |
Isotype | IgG2a |
Immunogen | Recombinant human TUBB protein |
Abbre | beta Tubulin |
Synonyms | Beta 5 tubulin, Beta1 tubulin, Class I beta tubulin, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, Tubulin beta 2 chain, Tubulin beta chain, Tubulin beta class I, beta Ib tubulin, tubulin be, tubulin beta 1 chain, tubulin beta 5 chain, Beta 4 tubulin |
Swissprot | |
Calculated MW | 55 kDa |
Observed MW | 55 kDa |
Cellular Localization | Cytoplasm, Cytoskeleton, Microtubule. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Clone No. | 3A7 |
Conjugation | Unconjugated |
Background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
Western blotting with anti-Beta Tubulin monoclonal antibody at dilution of 1:5000.
Lane 1: Hela cell lysates, Lane 2: Caco-2 cell lysates, Lane 3: HT-29 cell lysates, Lane 4: NIH/3T3 cell lysates, Lane 5:Raw264.7 cell lysates, Lane 6: Rat liver
Immunofluorescent analysis of HEK293 cells with anti-beta Tubulin monoclonal antibody at dilution of 1:4000.
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