CA9 Monoclonal Antibody-Elabscience伊莱瑞特【官网】

CA9 Monoclonal Antibody

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货号：E-AB-22034
说明书

说明书

Price: ¥2310.00 ¥1420.00 ¥580.00

Size:

120 μL 60 μL 20 μL

宿主： Mouse
反应性： Human
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: Hela, 293T Verified Samples in IHC: Human lung cancer Verified Samples in IF: Human liver cancer
Dilution	WB 1:1000-1:3000, IHC 1:50-300, IF 1:50-1:200
Clonality	Monoclonal
Isotype	IgG
Immunogen	Synthetic Peptide
Abbre	CA IX
Synonyms	CA-IX, CA9, CAH9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, MN, Membrane antigen MN, P54/58N, RCC associated protein G250, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250, pMW1
Swissprot	Q16790
Calculated MW	50 kDa
Observed MW	35-38 kDa
Cellular Localization	Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Tissue Specificity	Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Cardiovascular, Metabolism, Signal Transduction
Clone No.	9B5
Conjugation	Unconjugated
Background	Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Western Blot analysis of 1) Hela, 2) 293T cells using CA9 Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded Human lung cancer tissue using CA9 Monoclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Human liver cancer tissue using CA9 Monoclonal Antibody at dilution of 1:200.

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays