Calcein AM/PI Double Staining Kit
中文名称：Calcein AM/PI活死细胞双染试剂盒
Price:
- 应用： 细胞活力/细胞毒性
| 检测原理 | Elabscience® Calcein AM/PI Double Staining Kit可用于区分含有酯酶活性的哺乳动物的死细胞和活细胞，Calcein AM 是在传统的Calcein上添加上乙酰甲氧基甲酯即（AM）基团，疏水性增加，能很容易穿透活细胞膜，进入细胞内。Calcein AM本身无荧光，进入细胞后被细胞中内源性酯酶水解，生成具有强负电荷且不能通过细胞膜的极性分子Calcein滞留在细胞内，而Calcein可发出强绿色荧光（Ex/Em= 494nm/517nm）。由于死细胞缺乏酯酶，不能或很少能产生Calcein，因此仅活细胞会被染色为强绿色荧光，死细胞不能被染色或者染色非常弱。死细胞的细胞膜选择透过性丧失，碘化丙啶（Propidium Iodide，PI）可以进入胞内与双链DNA特异性结合并产生强烈的红色荧光（Ex/Em=535nm/617nm），从而对死细胞进行标记。因此，Calcein AM与碘化丙啶联合使用，可以对活细胞与死细胞同时进行双重荧光染色，可用于细胞活性与细胞毒性的检测。 |
| 检测方法 | 荧光法;流式 |
| 样本类型 | 细胞 |
| 检测时间 | 30 min |
| 检测仪器 | 流式细胞仪;荧光显微镜 |
| 荧光 | Calcein/PI |
| Ex/Em | 494/517,535/617 |
| Channel set | FITC, PE/PerCP/Cy5.5 |
| Filter set | FITC, TRITC |
| 自备试剂 | PBS(E-BC-R187) |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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| E-CK-A153 | Calcein AM Assay Buffer | 100mL |
| E-CK-A164 | Calcein AM Solution (100 μM) | 500Tests , 100Tests , 500Tests×10 |
| E-CK-A165 | Propidium Iodide (PI) Solution(750 μM) | 500Tests , 100Tests , 500Tests×10 |
