Calcein AM/PI Double Staining Kit

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中文名称：Calcein AM/PI活死细胞双染试剂盒

货号：E-CK-A354
文献（49）
说明书

说明书

Price: ¥2000.00 ¥700.00 ¥300.00

Size:

2000 Assays 500 Assays 100 Assays

应用： 细胞活力/细胞毒性

检测原理	Elabscience^® Calcein AM/PI Double Staining Kit可用于区分含有酯酶活性的哺乳动物的死细胞和活细胞，Calcein AM 是在传统的Calcein上添加上乙酰甲氧基甲酯即（AM）基团，疏水性增加，能很容易穿透活细胞膜，进入细胞内。Calcein AM本身无荧光，进入细胞后被细胞中内源性酯酶水解，生成具有强负电荷且不能通过细胞膜的极性分子Calcein滞留在细胞内，而Calcein可发出强绿色荧光（Ex/Em= 494nm/517nm）。由于死细胞缺乏酯酶，不能或很少能产生Calcein，因此仅活细胞会被染色为强绿色荧光，死细胞不能被染色或者染色非常弱。死细胞的细胞膜选择透过性丧失，碘化丙啶（Propidium Iodide，PI）可以进入胞内与双链DNA特异性结合并产生强烈的红色荧光（Ex/Em=535nm/617nm），从而对死细胞进行标记。因此，Calcein AM与碘化丙啶联合使用，可以对活细胞与死细胞同时进行双重荧光染色，可用于细胞活性与细胞毒性的检测。
检测方法	荧光法;流式
样本类型	细胞
检测时间	30 min
检测仪器	流式细胞仪;荧光显微镜
荧光	Calcein/PI
Ex/Em	494/517,535/617
Channel set	FITC, PE/PerCP/Cy5.5
Filter set	FITC, TRITC
自备试剂	PBS(E-BC-R187)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

4T1 cells and Hela cells were treated with 5 % DMSO, 5μM Camptothecin, 10μM Camptothecin for 4h, then stained with Calcein-AM / PI Double Staining Kit and photographed.

Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.

Jurkat cells were placed at 4 °C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.

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E-CK-A164	Calcein AM Solution (100 μM)	500 Tests , 100 Tests , 500 Tests × 10
E-CK-A165	Propidium Iodide (PI) Solution(750 μM)	500 Tests , 100 Tests , 500 Tests × 10
E-CK-A153	Calcein AM Assay Buffer	100 mL