CASP8 Monoclonal Antibody-Elabscience伊莱瑞特【官网】

CASP8 Monoclonal Antibody

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货号：E-AB-22107
文献（5）
说明书

说明书

Price: ¥2310.00 ¥1420.00 ¥580.00

Size:

120 μL 60 μL 20 μL

宿主： Mouse
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: Hela, Mouse brain, Rat brain Verified Samples in IHC: Mouse spleen Verified Samples in IF: Mouse liver
Dilution	WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant Protein
Abbre	Caspase-8
Synonyms	ALPS2B, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, Apoptotic cysteine protease, Apoptotic protease Mch-5, Apoptotic protease Mch5, CAP4, CASP-8, CASP8, Caspase 8, Caspase 8 apoptosis related cysteine peptidase, Caspase-8 sub
Swissprot	Q14790
Observed MW	43 ,57kDa
Cellular Localization	Cytoplasm.
Tissue Specificity	Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Cell Biology, Metabolism
Clone No.	2E3
Conjugation	Unconjugated
Background	Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Western Blot analysis of 1) Hela, 2) Mouse brain, 3) Rat brain with CASP8 Monoclonal Antibody

Immunohistochemistry of paraffin-embedded Mouse spleen tissue with CASP8 Monoclonal Antibody

Immunofluorescence analysis of Mouse liver tissue using CASP8 Monoclonal Antibody at dilution of 1:200.

Sorafenib and edaravone protect against renal fibrosis induced by unilateral ureteral obstruction via inhibition of oxidative stress, inflammation, and RIPK-3/MLKL pathway

IF: 3.100

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)

DOI：10.1007/s00210-024-03146-z

Reactivity：Rat

Sample Type：left kidney tissue
Allyl Isothiocyanate (AITC) Induces Apoptotic Cell Death In Vitro and Exhibits Anti-Tumor Activity in a Human Glioblastoma GBM8401/luc2 Model

IF: 6.208

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

DOI：10.3390/ijms231810411

PMID：36142326

Reactivity：Mouse

Sample Type：Tumor, heart, spleen, kidney, and liver
Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice

IF: 3.628

Journal： JOURNAL OF APPLIED TOXICOLOGY (2022)

DOI：10.1002/jat.4286

PMID：35001415

Reactivity：Mouse

Sample Type：lungs,liver
Suppression of EGFR/PKC-δ/NF-κB Signaling Associated With Imipramine-Inhibited Progression of Non-Small Cell Lung Cancer

IF: 6.244

Journal： Frontiers in Oncology (2021)

DOI：10.3389/fonc.2021.735183

PMID：34765548

Reactivity：Mouse

Sample Type：CL1-5-F4 cell-xenograft
The anti-cancer effect of flaxseed lignan derivatives on different acute myeloid leukemia cancer cells

IF: 4.545

Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)

DOI：10.1016/j.biopha.2020.110884

PMID：33080470

Reactivity：Human

Sample Type：Cell sample

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor^® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor^® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays

技术资源

• 如何明智选择抗体？

• 二抗选择指南

• Western Blot内参抗体选择原则

• 免疫组化抗体选择要点

• Western实验步骤

• Western Blot常见问题分析

• 不同浓缩胶体积所对应的各种组分的取样量

• 石蜡切片免疫组化实验步骤

• 免疫荧光实验指南

• 免疫共沉淀实验指南

全部分类

CASP8 Monoclonal Antibody

Sorafenib and edaravone protect against renal fibrosis induced by unilateral ureteral obstruction via inhibition of oxidative stress, inflammation, and RIPK-3/MLKL pathway

Allyl Isothiocyanate (AITC) Induces Apoptotic Cell Death In Vitro and Exhibits Anti-Tumor Activity in a Human Glioblastoma GBM8401/luc2 Model

Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice

Suppression of EGFR/PKC-δ/NF-κB Signaling Associated With Imipramine-Inhibited Progression of Non-Small Cell Lung Cancer

The anti-cancer effect of flaxseed lignan derivatives on different acute myeloid leukemia cancer cells

实验操作视频

技术资源