Caspase 8 Activity Assay Kit(Colorimetric Method)
中文名称：Caspase 8活性检测试剂盒(分光光度法)
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- 应用： 细胞凋亡-Caspase
| 检测原理 | Caspase 8又名FLICE、MACH和Mch5，Caspase 8可以自我活化，也可以在颗粒酶B的剪切下活化。Caspase 8特异性识别的序列是IETD，活化的caspase 8不但可以剪切PARP，而且还参与其它ICE家族蛋白酶，如caspase 3和caspase 7的活化过程。本caspase 8活性检测试剂盒是基于caspase 8可以催化底物Ac-IETD-pNA产生黄色的pNA(p-nitroaniline)，pNA在405nm附近有强吸收，从而可以通过测定吸光度来检测caspase 8的活性。 |
| 检测方法 | 比色法 |
| 样本类型 | 细胞;组织 |
| 检测时间 | 4-6 hours |
| 检测仪器 | 分光光度计 |
| 自备试剂 | PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M) |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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