Caspase 9 Activity Assay Kit(Colorimetric Method)
中文名称：Caspase 9活性检测试剂盒(分光光度法)
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- 应用： 细胞凋亡-Caspase
| 检测原理 | Caspase 9又称ICE-LAP6 或Mch6，是细胞凋亡信号转导过程中比较上游的一个caspase。线粒体释放细胞色素c以后，Caspase 9可以和细胞色素c以及Apaf1形成复合物，同时被激活。激活的caspase 9可以激活细胞凋亡的最关键酶caspase 3，从而促进后续的细胞凋亡信号。Caspase 9的激活可以通过磷酸化进行调控。本caspase 9活性检测试剂盒是基于caspase 9可以催化底物Ac-LEHD-pNA产生黄色的pNA(p-nitroaniline)，pNA在405nm附近有强吸收，从而可以通过测定吸光度来检测caspase 9的活性。 |
| 检测方法 | 比色法 |
| 样本类型 | 细胞;组织 |
| 检测时间 | 4-6 hours |
| 检测仪器 | 分光光度计 |
| 自备试剂 | PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M) |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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