Caspase 9 Activity Assay Kit(Colorimetric Method)

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中文名称：Caspase 9活性检测试剂盒(分光光度法)

货号：E-CK-A389
文献（23）
说明书

说明书

Caspase 9 Activity Assay Kit(Colorimetric Method)

Price: ¥1800.00 ¥1000.00 ¥500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Caspase

检测原理	Caspase 9又称ICE-LAP6 或Mch6，是细胞凋亡信号转导过程中比较上游的一个caspase。线粒体释放细胞色素c以后，Caspase 9可以和细胞色素c以及Apaf1形成复合物，同时被激活。激活的caspase 9可以激活细胞凋亡的最关键酶caspase 3，从而促进后续的细胞凋亡信号。Caspase 9的激活可以通过磷酸化进行调控。本caspase 9活性检测试剂盒是基于caspase 9可以催化底物Ac-LEHD-pNA产生黄色的pNA(p-nitroaniline)，pNA在405nm附近有强吸收，从而可以通过测定吸光度来检测caspase 9的活性。
检测方法	比色法
样本类型	细胞;组织
检测时间	4-6 hours
检测仪器	分光光度计;酶标仪
自备试剂	PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

pNA Standard Curve

MGST1 overexpression ameliorates mitochondrial dysfunction and ferroptosis during myocardial ischemia/reperfusion injury after heart transplantation

IF: 7.7

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)

DOI：10.1016/j.ijbiomac.2025.140135

Reactivity：Mouse

Sample Type：heart graft tissue
Exploring the Internal Environmental Changes of Muscle Cells and Apoptotic Phase of Mitochondria in Dry-Cured Loin Using Electrical Stimulation: Promoting the Precise Regulation of Loin Ham Quality

IF: 5.7

Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2025)

DOI：10.1021/acs.jafc.4c09673

Reactivity：Pig

Sample Type：meat tissue
Shikonin Ameliorates Rotenone-Induced Neurotoxicity Through Inhibition of Apoptosis via IGF-1R/PI3K/AKT Pathway in a Parkinson’s Disease-Associated SH-SY5Y Cell Model

IF: 4.6

Journal： MOLECULAR NEUROBIOLOGY (2025)

DOI：10.1007/s12035-025-04810-y

Reactivity：Human
Deciphering the effects of bixin on pulmonary alveolar adenocarcinoma migration and proliferation via targeting BAX/BCL-2 and Cyclin D1

IF: 3.8

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-96788-9

Reactivity：Human
Anti-metastatic potential of OR3 pigment from Streptomyces coelicolor JUACT03 on MDA-MB-231 breast cancer in vitro and in vivo

IF: 1.7

Journal： Asian Pacific Journal of Tropical Biomedicine (2025)

DOI：10.4103/apjtb.apjtb_623_24

Reactivity：Human
Ascorbic acid mitigates ROS-induced chilling injury development in postharvest sweet basil via improving antioxidant defense system to impede senescence process

IF: 6.4

Journal： POSTHARVEST BIOLOGY AND TECHNOLOGY (2024)

DOI：10.1016/j.postharvbio.2024.113265
Silencing of maternally expressed RNAs in Dlk1-Dio3 domain causes fatal vascular injury in the fetal liver

IF: 6.2

Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)

DOI：10.1007/s00018-024-05462-2
Recombinant Arginine Deiminase from Levilactobacillus brevis Inhibits the Growth of Stomach Cancer Cells, Possibly by Activating the Intrinsic Apoptosis Pathway

IF: 4.9

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms25084163
Intraperitoneal Injection of the Porphyromonas gingivalis Outer Membrane Vesicle (OMV) Stimulated Expressions of Neuroinflammatory Markers and Histopathological Changes in the Brains of Adult Zebrafish

IF: 4.9

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms252011025

Reactivity：Zebrafish

Sample Type：brain tissue
Anti-cancer activity of Brucine loaded Cu(OH)2/ZnO Nanoparticles Against Chronic Myeloid Leukemia Cell Line (K562) via apoptotic signaling Pathway

IF: 4.5

Journal： JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2024)

DOI：10.1016/j.jddst.2024.106159

Reactivity：Human

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