Flag-Tag Monoclonal Antibody-Elabscience伊莱瑞特【官网】

Flag-Tag Monoclonal Antibody

一键复制产品信息

货号：E-AB-48025
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： All
应用性： WB;IF;IP;FCM

Verified Samples	Verified Samples in WB: HEK293 Verified Samples in IF: 293F Verified Samples in IP: HEK-293 Verified Samples in FCM: CHO
Dilution	WB 1:5000-10000, IF 1:500-2000, IP 3μg/sample, FCM 0.2μg
Clonality	Monoclonal
Isotype	IgG2a
Immunogen	Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
Abbre	Flag-Tag
Synonyms	DDDDK epitope tag, DYKDDDDK epitope tag
Calculated MW	45 kDa
Observed MW	45 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS, pH 7.2. Contains 0.05% proclin 300.
Purification Method	Protein A/G Purification
Clone No.	3H9
Conjugation	Unconjugated
Background	FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.

Western blotting with anti-FLAG monoclonal antibody at dilution of 1:5000.lane 1: HEK 293 whole cell lysate, Lane 2: FLAG tag transfected HEK 293 cell lysates

Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Monoclonal Antibody at 1:2000 dilution.

Lane 1:HEK-293 transfected with DDDDK-tagged (input).Lane 2: Mouse monoclonal IgG2a instead of E-AB-48025 in HEK-293T transfected with DDDDK-tagged.Lane 3: E-AB-48025 IP in HEK-293T transfected with DDDDK-tagged.

1x10^6 CHO cells Transfected with a Flag plasmid were stained with 0.2ug Anti-Flag tag mAb(E-AB-48025) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays