GAPDH Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat ;Bovine;Caprine;Guinea pig;Rabbit;Porcine;
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB: DU145, OS-RC-2, T-47D, HEC-1B, HepG2, HEK-293, Rat heart, Porcine brain, Bovine kidney, Guinea pig liver, Rabbit pancreas, Caprine spleen Verified Samples in IF: 293F |
| Dilution | WB 1:5000-10000 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Abbre | GAPDH |
| Synonyms | BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW | 36 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cancer, Metabolism, Signal Transduction |
| Clone No. | 3B3 |
| Conjugation | Unconjugated |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR. |
Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000.
Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20g per lane.
Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000.
Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate.
Lysates/proteins at 20g per lane.
Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.
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