GAPDH Monoclonal Antibody（E-AB-48016）|-Elabscience伊莱瑞特【官网】

GAPDH Monoclonal Antibody

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货号：E-AB-48016
文献（35）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： Human;Mouse;Rat ;Bovine;Caprine;Guinea pig;Rabbit;Porcine;
应用性： WB;IF

Verified Samples	Verified Samples in WB: DU145, OS-RC-2, T-47D, HEC-1B, HepG2, HEK-293, Rat heart, Porcine brain, Bovine kidney, Guinea pig liver, Rabbit pancreas, Caprine spleen Verified Samples in IF: 293F
Dilution	WB 1:5000-10000
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human GAPDH protein expressed by E.coli
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	P04406
Calculated MW	36 kDa
Observed MW	36 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Cancer, Metabolism, Signal Transduction
Clone No.	3B3
Conjugation	Unconjugated
Background	GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.

Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000. Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20g per lane.

Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000. Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate. Lysates/proteins at 20g per lane.

Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.

Inhibition of lactate dehydrogenase A by diclofenac sodium induces apoptosis in HeLa cells through activation of AMPK

IF: 5.5

Journal： FEBS Journal (2024)

DOI：10.1111/febs.17158

Reactivity：Human
Formononetin protects against Aspergillus fumigatus Keratitis: Targeting inflammation and fungal load

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.112046

Reactivity：Mouse,Human
Synergistic effect of collagen peptide LR-7 and taurine on the prevention of cardiovascular injury in high salt-induced hypertensive mice

IF: 4.8

Journal： Food Bioscience (2024)

DOI：10.1016/j.fbio.2024.104907

Reactivity：mouse

Sample Type：hear
Transdermal Delivery of Recombinant Human Growth Hormone by Liposomal Gel for Skin Photoaging Therapy

IF: 4.6

Journal： ACS Applied Bio Materials (2024)

DOI：10.1021/acsabm.4c00391

Reactivity：Human
Computational and in?vitro screening validates the repositioning potential of Coxibs as anti-fibrotic agents

IF: 4.4

Journal： JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2024)

DOI：10.1080/07391102.2024.2318655

PMID：38433403

Reactivity：Human
Plumbagin inhibits fungal growth, HMGB1/LOX-1 pathway and inflammatory factors in A. fumigatus keratitis

IF: 4.0

Journal： Frontiers in Microbiology (2024)

DOI：10.3389/fmicb.2024.1383509

Reactivity：Mouse

Sample Type：cornea
Progranulin Protects against Aspergillus fumigatus Keratitis by Attenuating the Inflammatory Response through Enhancing Autophagy

IF: 4.0

Journal： ACS Infectious Diseases (2024)

DOI：10.1021/acsinfecdis.4c00236

Reactivity：Mouse

Sample Type：corneas tissue
Impact of macrophage differentiation on hematopoietic function enhancement by Shenzhu ErKang Syrup.

IF: 3.9

Journal： Aging-US (2024)

DOI：10.18632/aging.205358

Reactivity：Mouse

Sample Type：spleen tissue
LncRNA MIAT suppresses inflammation in LPS-induced J774A.1 macrophages by promoting autophagy through miR-30a-5p/SOCS1 axi

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-73607-1

Reactivity：Mouse
CISD3 is a prognostic biomarker and therapeutic target in pan-cancer

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-74247-1

Reactivity：Human

Sample Type：RCC tumor tissue,adjacent non-tumor tissue

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