GAPDH Monoclonal Antibody（E-AB-48016）|-Elabscience伊莱瑞特【官网】

GAPDH Monoclonal Antibody

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货号：E-AB-48016
文献（35）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： Human;Mouse;Rat ;Bovine;Caprine;Guinea pig;Rabbit;Porcine;
应用性： WB;IF

Verified Samples	Verified Samples in WB: DU145, OS-RC-2, T-47D, HEC-1B, HepG2, HEK-293, Rat heart, Porcine brain, Bovine kidney, Guinea pig liver, Rabbit pancreas, Caprine spleen Verified Samples in IF: 293F
Dilution	WB 1:5000-10000
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human GAPDH protein expressed by E.coli
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	P04406
Calculated MW	36 kDa
Observed MW	36 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Cancer, Metabolism, Signal Transduction
Clone No.	3B3
Conjugation	Unconjugated
Background	GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.

Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000. Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20g per lane.

Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000. Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate. Lysates/proteins at 20g per lane.

Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.

CD38 modulates cytokine secretion by NK cells through the Sirt1/NF-κB pathway, suppressing immune surveillance in colorectal cancer

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-79008-8

Reactivity：Human
Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

IF: 3.4

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e31653

Reactivity：Human
Cathelicidin boosts the antifungal activity of neutrophils and improves prognosis during Aspergillus fumigatus keratitis

IF: 2.9

Journal： INFECTION AND IMMUNITY (2024)

DOI：10.1128/iai.00483-23

Reactivity：Human,Mouse

Sample Type：cornea tissue
Targeting POLD1 to suppress the proliferation and migration of breast cancer MDA-MB-231 cell lines by downregulation of SIRT1

IF: 2.2

Journal： Toxicology Research (2024)

DOI：10.1093/toxres/tfae111

Reactivity：Human
Ultrasound-stimulated microbubbles enhances radiosensitivity in cervical cancer

IF: 2.1

Journal： INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2024)

DOI：10.1080/09553002.2024.2374903

Reactivity：Human
Wilms’ Tumor 1-Associating Protein Promotes Nonsmall-Cell Lung Cancer Through the Expression of Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5

IF: 1.6

Journal： AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS (2024)

DOI：10.1097/COC.0000000000001116

Reactivity：Human
Targeting Nrf2 with 3?H-1,2-dithiole-3-thione to moderate OXPHOS-driven oxidative stress attenuates IL-17A-induced psoriasis

IF: 7.500

Journal： BIOMEDICINE & PHARMACOTHERAPY (2023)

DOI：10.1016/j.biopha.2023.114294

PMID：36706632

Reactivity：Human
Persulfidation of mitoKv7.4 channels contributes to the cardioprotective effects of the H2S-donor Erucin against ischemia/reperfusion injury

IF: 5.8

Journal： BIOCHEMICAL PHARMACOLOGY (2023)

DOI：10.1016/j.bcp.2023.115728

PMID：37524208

Reactivity：Rat

Sample Type：heart tissue
RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer

IF: 5.400

Journal： Marine Drugs (2023)

DOI：10.3390/md21030151

PMID：36976201

Reactivity：Human
Pentoxifylline treats Aspergillus fumigatus keratitis by reducing fungal burden and suppressing corneal inflammation

IF: 5.000

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2023)

DOI：10.1016/j.ejphar.2023.175607

PMID：36822458

Reactivity：Mouse

Sample Type：corneas

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E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10 mL , 3 mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10 mL , 3 mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays