GAPDH Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody (无库存)

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货号：E-AB-40337
文献（110）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Dopamine reuptake and inhibitory mechanisms in human dopamine transporter

IF: 50.500

Journal： NATURE (2024)

DOI：10.1038/s41586-024-07796-0

Reactivity：Human
Nanoplastics induced health risk: Insights into intestinal barrier homeostasis and potential remediation strategy by dietary intervention

IF: 12.200

Journal： JOURNAL OF HAZARDOUS MATERIALS (2024)

DOI：10.1016/j.jhazmat.2024.134509

Reactivity：Mouse

Sample Type：intestine tissue
Tumor-Secreted Extracellular Vesicles Counteract Therapy Response by Triggering Inflammatory Mesenchymal Stem Cell Development

IF: 10.000

Journal： CLINICAL CANCER RESEARCH (2024)

DOI：10.1158/1078-0432.CCR-23-4097

Reactivity：Human
CircMLH3 induces mononuclear macrophage pyroptosis in sepsis by sponging miR-590-3p to regulate TAK1 expression

IF: 8.200

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.130179

PMID：38378118

Reactivity：Mouse,Human

Sample Type：lung tissue
Protective effects of olive oil antioxidant phenols on mercury-induced phosphatidylserine externalization in erythrocyte membrane: Insights into scramblase and flippase activity

IF: 7.100

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2024)

DOI：10.1016/j.freeradbiomed.2024.11.047

Reactivity：Human
NLRC5 exerts anti-endometriosis effects through inhibiting ERβ-mediated inflammatory response

IF: 7.000

Journal： BMC Medicine (2024)

DOI：10.1186/s12916-024-03571-0

Reactivity：Mouse,Human

Sample Type：mouse eutopic tissue,ectopic endometria tissue
Oleanolic acid 28-O-β-D-glucopyranoside: A novel therapeutic agent against ulcerative colitis via anti-inflammatory, barrier-preservation, and gut microbiota-modulation

IF: 6.900

Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)

DOI：10.1016/j.biopha.2024.117534

Reactivity：Mouse

Sample Type：colon tissue
Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

IF: 6.100

Journal： Neural Regeneration Research (2024)

DOI：10.4103/NRR.NRR-D-23-01355

PMID：38526285

Reactivity：Human
Proline Dehydrogenase (PRODH) Is Expressed in Lung Adenocarcinoma and Modulates Cell Survival and 3D Growth by Inducing Cellular Senescence

IF: 5.600

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms25020714

PMID：38255788

Reactivity：Human
Alginate oligosaccharide alleviates vascular aging by upregulating glutathione peroxidase 7

IF: 5.600

Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2024)

DOI：10.1016/j.jnutbio.2024.109578

PMID：38216066

Reactivity：Rat

Sample Type：aortas

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