GAPDH Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody (无库存)

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货号：E-AB-40337
文献（110）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

K63 ubiquitin chains target NLRP3 inflammasome for autophagic degradation in ox-LDL-stimulated THP-1 macrophages

IF: 4.831

Journal： Aging-US (2020)

DOI：10.18632/aging.102710

PMID：32003754

Reactivity：Human

Sample Type：Cell culture supernatant
FCN-A mediates the inflammatory response and the macrophage polarization in Aspergillus fumigatus keratitis of mice by activating the MAPK signaling pathway

IF: 3.943

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)

DOI：10.1016/j.intimp.2020.106473

PMID：32272397

Reactivity：Mouse

Sample Type：Cell lysate,Tissue homogenate
The Role of Autophagy in the Innate Immune Response to Fungal Keratitis Caused by Aspergillus fumigatus Infection

IF: 3.470

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020)

DOI：10.1167/iovs.61.2.25

Reactivity：Mouse

Sample Type：cornea tissue
Lpl-C310R mutation is associated with impaired glucose tolerance and endoplasmic reticulum stress in skeletal muscle

IF: 2.985

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)

DOI：10.1016/j.bbrc.2020.06.055

PMID：32703455

Reactivity：Mouse

Sample Type：Plasma
Effect of Insulin-Regulated FOXC2 Expression in Adipocyte Differentiation and Insulin Resistance

IF: 2.842

Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020)

DOI：10.2147/DMSO.S262950

PMID：32821143

Reactivity：Human

Sample Type：Tissue homogenate
Fermented Unpolished Black Rice (Oryza sativa L.) Inhibits Melanogenesis via ERK, p38, and AKT Phosphorylation in B16F10 Melanoma Cells

IF: 1.992

Journal： JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2020)

DOI：10.4014/jmb.2003.03019

PMID：32423183

Reactivity：Mouse

Sample Type：Cell sample
Ginsenoside Rg3 inhibits the biological activity of SGC-7901

IF: 1.797

Journal： Food Science & Nutrition (2020)

DOI：10.1002/fsn3.1707

PMID：32884696

Reactivity：Human

Sample Type：Cell lysate
Regulation of LOX-1 on adhesion molecules and neutrophil infiltration in mouse Aspergillus fumigatus keratitis

IF: 1.330

Journal： International Journal of Ophthalmology (2020)

DOI：10.18240/ijo.2020.06.03

PMID：32566496

Reactivity：Mouse

Sample Type：Tissue homogenate,Tissue Slice
Collagen receptor cross-talk determines α-smooth muscle actin-dependent collagen gene expression in angiotensin II–stimulated cardiac fibroblasts

IF: 4.106

Journal： JOURNAL OF BIOLOGICAL CHEMISTRY (2019)

DOI：10.1074/jbc.RA119.009744

PMID：31699892

Reactivity：Rat
Biological effects on tooth root surface topographies induced by various mechanical treatments

IF: 3.973

Journal： COLLOIDS AND SURFACES B-BIOINTERFACES (2019)

DOI：10.1016/j.colsurfb.2019.110748

PMID：31884082

Reactivity：Human

Sample Type：Gingival Crevicular Fluid

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