GAPDH Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody (无库存)

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货号：E-AB-40337
文献（110）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

LPS induces CXCL16 expression in HUVECs through the miR-146a-mediated TLR4 pathway

IF: 3.361

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2019)

DOI：10.1016/j.intimp.2019.01.011

PMID：30710793

Reactivity：Human

Sample Type：Cell culture supernate,Cell lysate
The role of netrin-1 in the mouse cornea during Aspergillus fumigatus infection

IF: 3.361

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2019)

DOI：10.1016/j.intimp.2019.03.047

PMID：30952101

Reactivity：Mouse

Sample Type：Tissue homogenate,Cell lysate
Role of gingival mesenchymal stem cell exosomes in macrophage polarization under inflammatory conditions

IF: 3.361

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2019)

DOI：10.1016/j.intimp.2019.106030

PMID：31796385

Reactivity：Human
Lipid-lowering and antioxidative effects of Apium graveolens L. root flavonoid extracts

IF: 3.049

Journal： RSC Advances (2019)

DOI：10.1039/C9RA04481G

PMID：35528576

Reactivity：Rat

Sample Type：liver, aortic
PI3K/Akt signaling pathway may be involved in MCP-1-induced P2X4R expression in cultured microglia and cancer-induced bone pain rats

IF: 2.173

Journal： NEUROSCIENCE LETTERS (2019)

DOI：10.1016/j.neulet.2019.02.024

PMID：30776493

Reactivity：Rat

Sample Type：Tissue homogenate
Pannexin 1 Channels Contribute to IL-1β Expression via NLRP3/Caspase-1 Inflammasome in Aspergillus Fumigatus Keratitis

IF: 1.672

Journal： CURRENT EYE RESEARCH (2019)

DOI：10.1080/02713683.2019.1584321

PMID：30793631

Reactivity：Mouse

Sample Type：Tissue homogenate
Doxycycline inhibits NAcht Leucine-rich repeat Protein 3 inflammasome activation and interleukin-1β production induced by Porphyromonas gingivalis-lipopolysaccharide and adenosine triphosphate in human gingival fibroblasts

IF: 1.663

Journal： ARCHIVES OF ORAL BIOLOGY (2019)

DOI：10.1016/j.archoralbio.2019.104514

PMID：31394382

Reactivity：Human

Sample Type：Cell culture supernatant
Disparate expression of autophagy in corneas of C57BL/6 mice and BALB/c mice after Aspergillus fumigatus infection

IF: 1.189

Journal： International Journal of Ophthalmology (2019)

DOI：10.18240/ijo.2019.05.02

PMID：31131226

Reactivity：Mouse

Sample Type：corneas
ATPR-induced G0/G1 phase arrest in gastric cancer cells by regulating the binding of 14-3-3ε and filamin A

IF: 3.202

Journal： Cancer Medicine (2018)

DOI：10.1002/cam4.1583

PMID：29862660

Reactivity：Human

Sample Type：Cell lysate
Astragaloside IV inhibits pathological functions of gastric cancer-associated fibroblasts

IF: 3.365

Journal： WORLD JOURNAL OF GASTROENTEROLOGY (2017)

DOI：10.3748/wjg.v23.i48.8512

PMID：29358859

Reactivity：Human

Sample Type：Cell lysate

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