GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Revealing the mechanism of “Huai Hua San” in the treatment of ulcerative colitis based on network pharmacology and experimental study

IF: 4.360

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2021)

DOI：10.1016/j.jep.2021.114321

PMID：34118340

Reactivity：Mouse

Sample Type：colon
Alginate Oligosaccharide Ameliorates D-Galactose-Induced Kidney Aging in Mice through Activation of the Nrf2 Signaling Pathway

IF: 3.411

Journal： Biomed Research International (2021)

DOI：10.1155/2021/6623328

PMID：33506023

Reactivity：Mouse

Sample Type：Kidney tissue
An inquest into regulatory mechanism of caveolin by ischemic preconditioning against orchidectomy-challenged rat heart

IF: 3.396

Journal： MOLECULAR AND CELLULAR BIOCHEMISTRY (2021)

DOI：10.1007/s11010-021-04109-1

PMID：33646465

Reactivity：Rat

Sample Type：heart
IL-6/STAT3 pathway is involved in the regulation of autophagy in chronic non-bacterial prostatitis cells, and may be affected by the NLRP3 inflammasome

IF: 1.094

Journal： ULTRASTRUCTURAL PATHOLOGY (2021)

DOI：10.1080/01913123.2021.1966149

Reactivity：Rat

Sample Type：prostate tissue
Strophanthidin Attenuates MAPK, PI3K/AKT/mTOR, and Wnt/β-Catenin Signaling Pathways in Human Cancers

IF: 4.848

Journal： Frontiers in Oncology (2020)

DOI：10.3389/fonc.2019.01469

PMID：32010609

Reactivity：Human
K63 ubiquitin chains target NLRP3 inflammasome for autophagic degradation in ox-LDL-stimulated THP-1 macrophages

IF: 4.831

Journal： Aging-US (2020)

DOI：10.18632/aging.102710

PMID：32003754

Reactivity：Human

Sample Type：Cell culture supernatant
FCN-A mediates the inflammatory response and the macrophage polarization in Aspergillus fumigatus keratitis of mice by activating the MAPK signaling pathway

IF: 3.943

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)

DOI：10.1016/j.intimp.2020.106473

PMID：32272397

Reactivity：Mouse

Sample Type：Cell lysate,Tissue homogenate
The Role of Autophagy in the Innate Immune Response to Fungal Keratitis Caused by Aspergillus fumigatus Infection

IF: 3.470

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020)

DOI：10.1167/iovs.61.2.25

Reactivity：Mouse

Sample Type：cornea tissue
Lpl-C310R mutation is associated with impaired glucose tolerance and endoplasmic reticulum stress in skeletal muscle

IF: 2.985

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)

DOI：10.1016/j.bbrc.2020.06.055

PMID：32703455

Reactivity：Mouse

Sample Type：Plasma
Effect of Insulin-Regulated FOXC2 Expression in Adipocyte Differentiation and Insulin Resistance

IF: 2.842

Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020)

DOI：10.2147/DMSO.S262950

PMID：32821143

Reactivity：Human

Sample Type：Tissue homogenate

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