GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Vagus nerve stimulation alleviates myocardial injury following hepatic ischemia–reperfusion in rats by inhibiting ferroptosis via the activation of the SLC7A11/GPX4 axis

IF: 2.8

Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2025)

DOI：10.1186/s40001-025-02416-7

Reactivity：Rat

Sample Type：ventricular myocardium tissue
Neuronal network inactivity potentiates neuropeptide release from mouse cortical neurons

IF: 2.7

Journal： eNeuro (2025)

DOI：10.1523/ENEURO.0555-24.2024

Reactivity：Mouse
Study on the mechanism of BGN in progression and metastasis of ccRCC

IF: 2.1

Journal： BMC Medical Genomics (2025)

DOI：10.1186/s12920-025-02124-5

Reactivity：Human
Dopamine reuptake and inhibitory mechanisms in human dopamine transporter

IF: 50.5

Journal： NATURE (2024)

DOI：10.1038/s41586-024-07796-0

Reactivity：Human
Nanoplastics induced health risk: Insights into intestinal barrier homeostasis and potential remediation strategy by dietary intervention

IF: 12.2

Journal： JOURNAL OF HAZARDOUS MATERIALS (2024)

DOI：10.1016/j.jhazmat.2024.134509

Reactivity：Mouse

Sample Type：intestine tissue
Tumor-Secreted Extracellular Vesicles Counteract Therapy Response by Triggering Inflammatory Mesenchymal Stem Cell Development

IF: 10.0

Journal： CLINICAL CANCER RESEARCH (2024)

DOI：10.1158/1078-0432.CCR-23-4097

Reactivity：Human
CircMLH3 induces mononuclear macrophage pyroptosis in sepsis by sponging miR-590-3p to regulate TAK1 expression

IF: 8.2

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.130179

PMID：38378118

Reactivity：Mouse,Human

Sample Type：lung tissue
Protective effects of olive oil antioxidant phenols on mercury-induced phosphatidylserine externalization in erythrocyte membrane: Insights into scramblase and flippase activity

IF: 7.1

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2024)

DOI：10.1016/j.freeradbiomed.2024.11.047

Reactivity：Human
NLRC5 exerts anti-endometriosis effects through inhibiting ERβ-mediated inflammatory response

IF: 7.0

Journal： BMC Medicine (2024)

DOI：10.1186/s12916-024-03571-0

Reactivity：Mouse,Human

Sample Type：mouse eutopic tissue,ectopic endometria tissue
Oleanolic acid 28-O-β-D-glucopyranoside: A novel therapeutic agent against ulcerative colitis via anti-inflammatory, barrier-preservation, and gut microbiota-modulation

IF: 6.9

Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)

DOI：10.1016/j.biopha.2024.117534

Reactivity：Mouse

Sample Type：colon tissue

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