GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

IF: 6.1

Journal： Neural Regeneration Research (2024)

DOI：10.4103/NRR.NRR-D-23-01355

PMID：38526285

Reactivity：Human
Proline Dehydrogenase (PRODH) Is Expressed in Lung Adenocarcinoma and Modulates Cell Survival and 3D Growth by Inducing Cellular Senescence

IF: 5.6

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms25020714

PMID：38255788

Reactivity：Human
Alginate oligosaccharide alleviates vascular aging by upregulating glutathione peroxidase 7

IF: 5.6

Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2024)

DOI：10.1016/j.jnutbio.2024.109578

PMID：38216066

Reactivity：Rat

Sample Type：aortas
Inhibition of lactate dehydrogenase A by diclofenac sodium induces apoptosis in HeLa cells through activation of AMPK

IF: 5.5

Journal： FEBS Journal (2024)

DOI：10.1111/febs.17158

Reactivity：Human
Trastuzumab-conjugated liposomes for co-delivery of paclitaxel and anti-abcb1 siRNA in HER2-positive breast cancer: In vitro and in vivo evaluations

IF: 5.0

Journal： JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2024)

DOI：10.1016/j.jddst.2024.105614

Reactivity：Human
Formononetin protects against Aspergillus fumigatus Keratitis: Targeting inflammation and fungal load

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.112046

Reactivity：Mouse,Human
Synergistic effect of collagen peptide LR-7 and taurine on the prevention of cardiovascular injury in high salt-induced hypertensive mice

IF: 4.8

Journal： Food Bioscience (2024)

DOI：10.1016/j.fbio.2024.104907

Reactivity：mouse

Sample Type：hear
Sheng Xue Ning as a Novel Agent that Promotes SCF-Driven Hematopoietic Stem/Progenitor Cell Proliferation to Promote Erythropoiesis

IF: 4.8

Journal： Biomolecules (2024)

DOI：10.3390/biom14091147

Reactivity：Mouse

Sample Type：spleen tissue
Near infrared-responsive quinacrine–gold hybrid nanoparticles deregulate HSP-70/P300-mediated H3K14 acetylation in ER/PR+ breast cancer stem cells

IF: 4.7

Journal： Nanomedicine (2024)

DOI：10.2217/nnm-2023-0269

Reactivity：人
Transdermal Delivery of Recombinant Human Growth Hormone by Liposomal Gel for Skin Photoaging Therapy

IF: 4.6

Journal： ACS Applied Bio Materials (2024)

DOI：10.1021/acsabm.4c00391

Reactivity：Human

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