GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Unraveling CCL20's role by regulating Th17 cell chemotaxis in experimental autoimmune prostatitis

IF: 4.3

Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2024)

DOI：10.1111/jcmm.18445

Reactivity：Mouse

Sample Type：prostate tissues
Lysophosphatidylcholine 14:0 Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Protecting Alveolar Epithelial Barrier by Activation of Nrf2/HO-1 Pathway

IF: 4.2

Journal： Journal of Inflammation Research (2024)

DOI：10.2147/JIR.S495227

Reactivity：Mouse

Sample Type：lung tissue
Molecular Editing of NSC-666719 Enabling Discovery of benzodithiazinedioxide-guanidines as Anticancer Agents

IF: 4.1

Journal： RSC Medicinal Chemistry (2024)

DOI：10.1039/D3MD00648D

PMID：38516586

Reactivity：Human
Plumbagin inhibits fungal growth, HMGB1/LOX-1 pathway and inflammatory factors in A. fumigatus keratitis

IF: 4.0

Journal： Frontiers in Microbiology (2024)

DOI：10.3389/fmicb.2024.1383509

Reactivity：Mouse

Sample Type：cornea
Progranulin Protects against Aspergillus fumigatus Keratitis by Attenuating the Inflammatory Response through Enhancing Autophagy

IF: 4.0

Journal： ACS Infectious Diseases (2024)

DOI：10.1021/acsinfecdis.4c00236

Reactivity：Mouse

Sample Type：corneas tissue
Chuanzhitongluo capsule improves cognitive impairment in mice with chronic cerebral hypoperfusion via the cholinergic anti-inflammatory pathway

IF: 3.9

Journal： EXPERIMENTAL GERONTOLOGY (2024)

DOI：10.1016/j.exger.2024.112407

PMID：38522309

Reactivity：Mouse

Sample Type：hippocampal tissue
LncRNA MIAT suppresses inflammation in LPS-induced J774A.1 macrophages by promoting autophagy through miR-30a-5p/SOCS1 axi

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-73607-1

Reactivity：Mouse
CD38 modulates cytokine secretion by NK cells through the Sirt1/NF-κB pathway, suppressing immune surveillance in colorectal cancer

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-79008-8

Reactivity：Human
Oxymatrine mitigates Aspergillus fumigatus keratitis by suppressing fungal activity and restricting pyroptosis

IF: 3.4

Journal： EXPERIMENTAL EYE RESEARCH (2024)

DOI：10.1016/j.exer.2024.109830

PMID：38364932

Reactivity：Mouse

Sample Type：corneas
Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

IF: 3.4

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e31653

Reactivity：Human

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