GAPDH Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody (无库存)

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货号：E-AB-40337
文献（110）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Palmitic Acid Inhibits the Growth and Metastasis of Gastric Cancer by Blocking the STAT3 Signaling Pathway

IF: 5.200

Journal： Cancers (2023)

DOI：10.3390/cancers15020388

PMID：36672337

Reactivity：Human
αV-Integrin-Dependent Inhibition of Glioblastoma Cell Migration, Invasion and Vasculogenic Mimicry by the uPAcyclin Decapeptide

IF: 5.200

Journal： Cancers (2023)

DOI：10.3390/cancers15194775

PMID：37835469

Reactivity：Human
Pentoxifylline treats Aspergillus fumigatus keratitis by reducing fungal burden and suppressing corneal inflammation

IF: 5.000

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2023)

DOI：10.1016/j.ejphar.2023.175607

PMID：36822458

Reactivity：Mouse

Sample Type：corneas
Novel HDAC inhibitors provide neuroprotection in MPTP-induced Parkinson's disease model of rats

IF: 5.000

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2023)

DOI：10.1016/j.ejphar.2023.176067

PMID：37751833

Reactivity：Rat

Sample Type：brain
Inhibition of TLR4 Alleviates Heat Stroke-Induced Cardiomyocyte Injury by Down-Regulating Inflammation and Ferroptosis

IF: 4.600

Journal： MOLECULES (2023)

DOI：10.3390/molecules28052297

PMID：36903542

Reactivity：Rat
A Coumarin–Imidazothiadiazole Derivative, SP11 Abrogates Tumor Growth by Targeting HSP90 and Its Client Proteins

IF: 4.600

Journal： MOLECULES (2023)

DOI：10.3390/molecules28135226

PMID：37446888

Reactivity：Human
M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

IF: 4.500

Journal： Journal of Inflammation Research (2023)

DOI：10.2147/JIR.S413358

PMID：37489150

Reactivity：Human
Quinacrine inhibits cMET-mediated metastasis and angiogenesis in breast cancer stem cells

IF: 4.100

Journal： Journal of Cell Communication and Signaling (2023)

DOI：10.1007/s12079-023-00756-9

PMID：37162635

Reactivity：Human
Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

IF: 4.000

Journal： Degenerative Neurological and Neuromuscular Disease (2023)

DOI：10.2147/DNND.S427781

PMID：37905186

Reactivity：Mouse

Sample Type：hippocampi
High-titer anti-interferon-γ neutralizing autoantibodies linked to opportunistic infections in patients with adult-onset still's disease

IF: 3.900

Journal： Frontiers in Medicine (2023)

DOI：10.3389/fmed.2022.1097514

PMID：36698819

Reactivity：Human

Sample Type：serum

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