GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Empagliflozin demonstrates cytotoxicity and synergy with tamoxifen in ER-positive breast cancer cells: anti-proliferative and anti-survival effects

IF: 3.1

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)

DOI：10.1007/s00210-024-03316-z

Reactivity：Human
Cathelicidin boosts the antifungal activity of neutrophils and improves prognosis during Aspergillus fumigatus keratitis

IF: 2.9

Journal： INFECTION AND IMMUNITY (2024)

DOI：10.1128/iai.00483-23

Reactivity：Human,Mouse

Sample Type：cornea tissue
Mesenchymal stem cell-derived exosomes modulate the COX-IV pathway via inhibition of amyloidogenesis and mitoprotection in sodium azide- Alzheimer model in rats.

IF: 2.7

Journal： Scientific African (2024)

DOI：10.1016/j.sciaf.2024.e02274

Reactivity：Rat

Sample Type：hippocampus tissue
Targeting POLD1 to suppress the proliferation and migration of breast cancer MDA-MB-231 cell lines by downregulation of SIRT1

IF: 2.2

Journal： Toxicology Research (2024)

DOI：10.1093/toxres/tfae111

Reactivity：Human
Ultrasound-stimulated microbubbles enhances radiosensitivity in cervical cancer

IF: 2.1

Journal： INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2024)

DOI：10.1080/09553002.2024.2374903

Reactivity：Human
Wilms’ Tumor 1-Associating Protein Promotes Nonsmall-Cell Lung Cancer Through the Expression of Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5

IF: 1.6

Journal： AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS (2024)

DOI：10.1097/COC.0000000000001116

Reactivity：Human
Supramolecular Integration of Multifunctional Nanomaterial by Mannose-Decorated Azocalixarene with Ginsenoside Rb1 for Synergistic Therapy of Rheumatoid Arthritis

IF: 17.1

Journal： ACS Nano (2023)

DOI：10.1021/acsnano.3c09140

PMID：38096153

Reactivity：Mouse
Quinacrine inhibits HIF-1α/VEGF-A mediated angiogenesis by disrupting the interaction between cMET and ABCG2 in patient-derived breast cancer stem cells

IF: 7.9

Journal： PHYTOMEDICINE (2023)

DOI：10.1016/j.phymed.2023.154914

PMID：37321076

Reactivity：Human
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons

IF: 7.7

Journal： eLife (2023)

DOI：10.7554/eLife.85561

PMID：37695731

Reactivity：Rat
Anti-Inflammatory and Anti-Oxidative Effects of Polysaccharides Extracted from Unripe Carica papaya L. Fruit

IF: 7.0

Journal： Antioxidants (2023)

DOI：10.3390/antiox12081506

PMID：37627501

Reactivity：Human

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