GAPDH Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody (无库存)

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货号：E-AB-40337
文献（110）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

2-Substituted 1,5-Benzothiazepine-based HDAC inhibitors exert anticancer activities on human solid and acute myeloid leukemia cell lines

IF: 3.500

Journal： BIOORGANIC & MEDICINAL CHEMISTRY (2023)

DOI：10.1016/j.bmc.2023.117444

PMID：37611334

Reactivity：Human

Sample Type：U937 cell,HCT116 cell
Caspase-3 and gasdermin E mediate macrophage pyroptosis in periodontitis

IF: 3.500

Journal： JOURNAL OF PERIODONTAL RESEARCH (2023)

DOI：10.1111/jre.13197

PMID：37885312

Reactivity：Human，Mouse

Sample Type：gingival tissues
Effect of hypobaric hypoxia on the fiber type transition of skeletal muscle: a synergistic therapy of exercise preconditioning with a nanocurcumin formulation

IF: 3.400

Journal： JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY (2023)

DOI：10.1007/s13105-023-00965-1

PMID：37147493

Reactivity：Rat

Sample Type：gastrocnemius
Different Proteins Regulated Apoptosis, Proliferation and Metastasis of Lung Adenocarcinoma After Radiotherapy at Different Time

IF: 3.300

Journal： Cancer Management and Research (2023)

DOI：10.2147/CMAR.S219967

PMID：32308480

Reactivity：Human

Sample Type：Cell lysate
Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin

IF: 2.500

Journal： ACTA HISTOCHEMICA (2023)

DOI：10.1016/j.acthis.2023.152030

PMID：37099996

Reactivity：Mouse

Sample Type：skin tissue
Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

IF: 8.786

Journal： Frontiers in Immunology (2022)

DOI：10.3389/fimmu.2022.915218

PMID：35860242

Reactivity：Mouse
Physicochemical properties and protective effects on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides by fractional precipitation

IF: 8.025

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

DOI：10.1016/j.ijbiomac.2022.12.254

PMID：36584774

Reactivity：Human
Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

IF: 7.675

Journal： Antioxidants (2022)

DOI：10.3390/antiox11040739

PMID：35453423

Reactivity：Human
Anti-PADI4 antibody suppresses breast cancer by repressing the citrullinated fibronectin in the tumor microenvironment

IF: 7.419

Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)

DOI：10.1016/j.biopha.2022.113289

PMID：35772376

Reactivity：Mouse

Sample Type：MDA-MB-231 cells-xenograft tumor tissue
Azetidin-2-one-based small molecules as dual hHDAC6/HDAC8 inhibitors: Investigation of their mechanism of action and impact of dual inhibition profile on cell viability

IF: 7.088

Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022)

DOI：10.1016/j.ejmech.2022.114409

PMID：35551034

Reactivity：Human

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