GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

A Coumarin–Imidazothiadiazole Derivative, SP11 Abrogates Tumor Growth by Targeting HSP90 and Its Client Proteins

IF: 4.6

Journal： MOLECULES (2023)

DOI：10.3390/molecules28135226

PMID：37446888

Reactivity：Human
M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

IF: 4.5

Journal： Journal of Inflammation Research (2023)

DOI：10.2147/JIR.S413358

PMID：37489150

Reactivity：Human
Quinacrine inhibits cMET-mediated metastasis and angiogenesis in breast cancer stem cells

IF: 4.100

Journal： Journal of Cell Communication and Signaling (2023)

DOI：10.1007/s12079-023-00756-9

PMID：37162635

Reactivity：Human
Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

IF: 4.0

Journal： Degenerative Neurological and Neuromuscular Disease (2023)

DOI：10.2147/DNND.S427781

PMID：37905186

Reactivity：Mouse

Sample Type：hippocampi
High-titer anti-interferon-γ neutralizing autoantibodies linked to opportunistic infections in patients with adult-onset still's disease

IF: 3.900

Journal： Frontiers in Medicine (2023)

DOI：10.3389/fmed.2022.1097514

PMID：36698819

Reactivity：Human

Sample Type：serum
2-Substituted 1,5-Benzothiazepine-based HDAC inhibitors exert anticancer activities on human solid and acute myeloid leukemia cell lines

IF: 3.5

Journal： BIOORGANIC & MEDICINAL CHEMISTRY (2023)

DOI：10.1016/j.bmc.2023.117444

PMID：37611334

Reactivity：Human

Sample Type：U937 cell,HCT116 cell
Caspase-3 and gasdermin E mediate macrophage pyroptosis in periodontitis

IF: 3.5

Journal： JOURNAL OF PERIODONTAL RESEARCH (2023)

DOI：10.1111/jre.13197

PMID：37885312

Reactivity：Human，Mouse

Sample Type：gingival tissues
Effect of hypobaric hypoxia on the fiber type transition of skeletal muscle: a synergistic therapy of exercise preconditioning with a nanocurcumin formulation

IF: 3.400

Journal： JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY (2023)

DOI：10.1007/s13105-023-00965-1

PMID：37147493

Reactivity：Rat

Sample Type：gastrocnemius
Different Proteins Regulated Apoptosis, Proliferation and Metastasis of Lung Adenocarcinoma After Radiotherapy at Different Time

IF: 3.300

Journal： Cancer Management and Research (2023)

DOI：10.2147/CMAR.S219967

PMID：32308480

Reactivity：Human

Sample Type：Cell lysate
Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin

IF: 2.500

Journal： ACTA HISTOCHEMICA (2023)

DOI：10.1016/j.acthis.2023.152030

PMID：37099996

Reactivity：Mouse

Sample Type：skin tissue

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