GAPDH Polyclonal Antibody（E-AB-40337）|-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody

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货号：E-AB-40337
文献（125）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

IF: 8.786

Journal： Frontiers in Immunology (2022)

DOI：10.3389/fimmu.2022.915218

PMID：35860242

Reactivity：Mouse
Physicochemical properties and protective effects on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides by fractional precipitation

IF: 8.025

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

DOI：10.1016/j.ijbiomac.2022.12.254

PMID：36584774

Reactivity：Human
Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts

IF: 7.675

Journal： Antioxidants (2022)

DOI：10.3390/antiox11040739

PMID：35453423

Reactivity：Human
Anti-PADI4 antibody suppresses breast cancer by repressing the citrullinated fibronectin in the tumor microenvironment

IF: 7.419

Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)

DOI：10.1016/j.biopha.2022.113289

PMID：35772376

Reactivity：Mouse

Sample Type：MDA-MB-231 cells-xenograft tumor tissue
Azetidin-2-one-based small molecules as dual hHDAC6/HDAC8 inhibitors: Investigation of their mechanism of action and impact of dual inhibition profile on cell viability

IF: 7.088

Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022)

DOI：10.1016/j.ejmech.2022.114409

PMID：35551034

Reactivity：Human
Novel pyridine-containing histone deacetylase inhibitors strongly arrest proliferation, induce apoptosis and modulate miRNAs in cancer cells

IF: 7.088

Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022)

DOI：10.1016/j.ejmech.2022.115022

PMID：36549114

Reactivity：Human
Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study

IF: 6.525

Journal： Pharmaceutics (2022)

DOI：10.3390/pharmaceutics15010007

PMID：36678636

Reactivity：Rat

Sample Type：brain
Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma

IF: 6.113

Journal： Frontiers in Molecular Biosciences (2022)

DOI：10.3389/fmolb.2022.822739

PMID：35372503

Reactivity：Human
High-density lipoprotein reduces microglia activation and protects against experimental autoimmune encephalomyelitis in mice

IF: 5.714

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2022)

DOI：10.1016/j.intimp.2022.108566

PMID：35101849

Reactivity：Mouse

Sample Type：spinal cord
Mechanism of antifungal activity and therapeutic action of β-ionone on Aspergillus fumigatus keratitis via suppressing LOX1 and JNK/p38 MAPK activation

IF: 5.714

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2022)

DOI：10.1016/j.intimp.2022.108992

PMID：35810488

Reactivity：Mouse

Sample Type：corneas

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