GAPDH Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GAPDH Polyclonal Antibody (无库存)

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货号：E-AB-40337
文献（110）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon
Dilution	WB 1:3000-1:6000, IHC 1:300-1:500
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein
Swissprot	Q5XJ10
Calculated MW	35 kDa
Observed MW	35 kDa
Cellular Localization	Cytoplasm, Cytoskeleton, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Conjugation	Unconjugated
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000

Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000

Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400

Alpha-Mangostin Reduces Pericellular Fibronectin on Suspended Tumor Cells and Therapeutically, but Not Prophylactically, Suppresses Distant Metastasis

IF: 3.251

Journal： Life-Basel (2022)

DOI：10.3390/life12091375

PMID：36143411

Reactivity：Mouse
Microglial activation and neurobiological alterations in experimental autoimmune prostatitis-induced depressive-like behavior in mice

IF: 2.989

Journal： Neuropsychiatric Disease and Treatment (2022)

DOI：10.2147/NDT.S211288

PMID：31496706

Reactivity：Mouse

Sample Type：Serum,Tissue homogenate
Paired related homeobox 1 attenuates autophagy via acetyl-CoA carboxylase 1-regulated fatty acid metabolism in salivary adenoid cystic carcinoma

IF: 2.792

Journal： FEBS Open Bio (2022)

DOI：10.1002/2211-5463.13367

PMID：35032368

Reactivity：Human
Perillaldehyde Protects Against Aspergillus fumigatus Keratitis by Reducing Fungal Load and Inhibiting Inflammatory Cytokines and LOX-1

IF: 2.555

Journal： CURRENT EYE RESEARCH (2022)

DOI：10.1080/02713683.2022.2093382

Reactivity：Mouse

Sample Type：corneas
Does Resveratrol Prevent Sevoflurane Toxicity in Newborn Rats?

IF: 2.542

Journal： JOURNAL OF MEDICINAL FOOD (2022)

DOI：10.1089/jmf.2021.0154

PMID：35420459

Reactivity：Rat

Sample Type：brain tissue
NADPH oxidase 2 plays a protective role in experimental Aspergillus fumigatus keratitis in mice through killing fungi and limiting the degree of inflammation

IF: 1.645

Journal： International Journal of Ophthalmology (2022)

DOI：10.18240/ijo.2022.07.02

PMID：35919314

Reactivity：Mouse

Sample Type：corneas
LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

IF: 10.711

Journal： Bioengineering & Translational Medicine (2021)

DOI：10.1002/btm2.10275

PMID：35600648

Reactivity：Human
Differential expression profiles of long noncoding RNAs and mRNAs in human bone marrow mesenchymal stem cells after exposure to a high dosage of dexamethasone

IF: 6.832

Journal： Stem Cell Research & Therapy (2021)

DOI：10.1186/s13287-020-02040-8

PMID：33407832

Reactivity：Human
Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease

IF: 6.081

Journal： Biomedicines (2021)

DOI：10.3390/biomedicines9111508

PMID：34829738

Reactivity：Human
LncRNA MALAT1 Facilitates Ovarian Cancer Progression through Promoting Chemoresistance and Invasiveness in the Tumor Microenvironment

IF: 5.924

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

DOI：10.3390/ijms221910201

PMID：34638541

Reactivity：Human

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