GFP Monoclonal Antibody-Elabscience伊莱瑞特【官网】

GFP Monoclonal Antibody

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货号：E-AB-48010
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： All
应用性： WB;IF;IP;FCM

Verified Samples	Verified Samples in WB: eGFP, eGFP protein with anti-eGFP Verified Samples in IF: zebrafish embryo Verified Samples in IP: 293 Verified Samples in FCM: CHO
Dilution	WB 1:100000-200000, IP 3μg/sample, IF 1:8000-16000, FCM 0.2μg
Clonality	Monoclonal
Isotype	IgG2a
Immunogen	Recombinant GFP protein expressed by E.coli
Abbre	GFP-Tag
Synonyms	GFP, Green fluorescent protein
Swissprot	P42212
Calculated MW	32 kDa
Observed MW	32 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS, pH 7.2. Contains 0.05% proclin 300.
Purification Method	Protein A/G Purification
Clone No.	5B5
Conjugation	Unconjugated
Background	The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization.

Western blot of eGFP protein with anti-eGFP at dilution of 1:80000.

Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.

Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:8000.

IP Result of 293 cells transfected with GFP-Tag fusion protein, using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody

1x10^6 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(E-AB-48010) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays