GFP Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: All
- 应用性: WB;IF;IP
Verified Samples |
Verified Samples in WB: 293F cells transfected with the GFP-tag Verified Samples in IP: 293F cells transfected with the FLAG-GFP, 293F, anti-GFP-Tag monoclonal antibody |
Dilution | WB 1:100000-200000, IF 1:8000-16000, IP 3ug/sample |
Clonality | Monoclonal |
Isotype | IgG2a |
Immunogen | Recombinant GFP protein expressed by E.coli |
Abbre | GFP-Tag |
Synonyms | GFP, Green fluorescent protein |
Swissprot | |
Calculated MW | 32 kDa |
Observed MW | 32 kDa |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Clone No. | 2A7 |
Conjugation | Unconjugated |
Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Western blot of GFP protein with anti-GFP at dilution of 1:50000, 1:100000, 200000. Lane 1:293F cells transfected with GFP-Tag fusion protein, lane 2: 293F cells transfected with GFP-Tag fusion protein, Lane 3:293F cells transfected with GFP-Tag fusion protein
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:50000.
IP Result of 293F cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
-
The glial UDP-glycosyltransferase Ugt35b regulates longevity by maintaining lipid homeostasis in Drosophila
IF: 7.500Journal: Cell Reports (2024)Reactivity:FlySample Type:Head tissue