HIF1 bata Monoclonal Antibody-Elabscience伊莱瑞特【官网】

HIF1 bata Monoclonal Antibody

一键复制产品信息

货号：E-AB-22189
说明书

说明书

Price: ¥2310.00 ¥1420.00 ¥580.00

Size:

120 μL 60 μL 20 μL

宿主： Mouse
反应性： Mouse
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: Mouse brain Verified Samples in IHC: Mouse brain
Dilution	WB 1:1000-2000, IHC 1:100-200
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant Protein
Abbre	HIF-1 β/ARNT
Synonyms	ARNT, ARNT protein, Aryl hydrocarbon receptor nuclear translocator, Class E basic helix-loop-helix protein 2, Dioxin receptor, Dioxin receptor nuclear translocator, Drnt, HIF 1 beta, HIF 1beta, HIF-1-beta, HIF1-beta, HIF1B, HIF1beta, Hypoxia Inducible Fact, arnT, bHLHe2
Swissprot	P27540
Observed MW	87 kDa
Cellular Localization	Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Clone No.	4C5
Conjugation	Unconjugated
Background	Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

Western Blot analysis of Mouse brain using HIF1 bata Monoclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Mouse brain tissue using HIF1 bata Monoclonal Antibody at dilution of 1:200.

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays