His-Tag Polyclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Rabbit
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: 6xHis-Tagged fused protein, HEK 293 |
| Dilution | WB 1:1000-2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Abbre | His-Tag |
| Synonyms | HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6His epitope tag, His tag |
| Calculated MW | 28 kDa |
| Observed MW | 28 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. It is the most widely used protein affinity tag. Due to its small size of only six amino acids it rarely in |
Western blot with anti-6xHis antibody at dilution of 1:2000.
lane 1: HEK 293 whole cell lysate, lane 2: 6xHis tag transfected HEK 293 cell lysates
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