mCherry-Tag Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: All
- 应用性: WB;IF
Verified Samples |
Verified Samples in WB: mCherry protein (27kDa) Verified Samples in IF: 293F |
Dilution | WB 1:1000-2000, IF 1:6000-12000 |
Clonality | Monoclonal |
Isotype | IgG1 |
Immunogen | Recombinant Discosoma sp. mCherry Protein |
Abbre | mCherry-Tag |
Synonyms | mCherry-Tag |
Swissprot | |
Calculated MW | 30 kDa |
Observed MW | 30 kDa |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Clone No. | 4E3 |
Conjugation | Unconjugated |
Background | mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish. mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments. |
Western blot of mCherry protein with anti-mCherry antibody at dilution of 1:2000.
Immunofluorescent analysis of 293F cells transfected with the mCherry, using anti-mCherry monoclonal antibody at dilution of 1:12000.