Mitochondrial Membrane Potential Assay Kit (with JC-1)
中文名称：线粒体膜电位检测试剂盒(JC-1)
Price:
- 应用： 细胞凋亡-JC-1
| 检测原理 | 线粒体膜电位下降是细胞凋亡早期的标志性事件，发生在细胞核凋亡特征（染色质浓缩、DNA断裂）出现之前，一旦线粒体膜电位崩溃，细胞凋亡便不可逆转。
JC-1是一种广泛用于检测线粒体膜电位 Ψm的理想荧光探针，可以检测细胞、组织或纯化的线粒体膜电位。JC-1有单体和多聚体两种存在形式，两者发射光谱不同。在正常细胞内，线粒体膜电位较高，JC-1聚集在线粒体的基质中，形成聚合物，可产生红色荧光；凋亡早期，线粒体膜电位降低，JC-1不能聚集在线粒体基质中，此时JC-1为单体，可产生绿色荧光。
通过JC-1从红色荧光到绿色荧光的转变可检测到细胞膜电位的下降，可将JC-1荧光颜色的转变作为细胞凋亡早期的检测指标。常用红绿荧光的相对比例来衡量线粒体去极化的比例。
JC-1单体的最大激发波长为514 nm，最大发射波长为529 nm；JC-1聚合物的最大激发波长为585 nm，最大发射波长为590 nm。实际观察时，常规设置红色荧光和绿色荧光即可。 |
| 检测方法 | 荧光法;流式 |
| 样本类型 | 细胞 |
| 检测时间 | 40min |
| 检测仪器 | 流式细胞仪;荧光显微镜 |
| 荧光 | JC-1 |
| Ex/Em | 514/529, 585/590 |
| Channel set | FITC, PE |
| 自备试剂 | 超纯水 |
| 保存温度 | 该产品可在2~8°C/-20°C避光条件下保存12个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
-
SP94 engineered erythrocyte membrane enhanced the targeted delivery of biomimetic nanosuspension with IDO immunotherapy and chemotherapy in liver cancer
IF: 13.300Journal： CHEMICAL ENGINEERING JOURNAL (2024) -
CaCO3-based nanomedicine with multi-functions of ferroptosis-apoptosis and microenvironment regulation in cancer
IF: 13.200 -
NIR-II light based combinatorial management of hypertrophic scar by inducing autophagy in fibroblasts
IF: 10.600Journal： JOURNAL OF NANOBIOTECHNOLOGY (2024) -
Multifunctional polymeric nanocapsules with enhanced cartilage penetration and retention for osteoarthritis treatment
IF: 10.500Journal： JOURNAL OF CONTROLLED RELEASE (2024) -
Metabolic Control During Macrophage Polarization by a Citrate-Functionalized Scaffold for Maintaining Bone Homeostasis
IF: 10.000Journal： Advanced Healthcare Materials (2024) -
Multifunctional Metal–Phenolic Composites Promote Efficient Periodontitis Treatment via Antibacterial and Osteogenic Properties
IF: 9.500 -
Deficiency of polypeptide N-acetylgalactosamine transferase 9 contributes to a risk for Parkinson's disease via mitochondrial dysfunctions
IF: 8.200Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)PMID：38401583 -
Identification of Cathepsin B as a Therapeutic Target for Ferroptosis of Macrophage after Spinal Cord Injury
IF: 7.400Journal： Aging and Disease (2024) -
Trehalose extricates impaired mitochondrial and autophagy dysregulation in patient iPSC-derived macular corneal dystrophy disease model
IF: 7.100Journal： Stem Cell Research & Therapy (2024) -
Discovery, Synthesis, and Activity Evaluation of Novel Five-Membered Sulfur-Containing Heterocyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo
IF: 6.800Journal： JOURNAL OF MEDICINAL CHEMISTRY (2024)
| E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100 Assays , 50 Assays , 20 Assays |
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20Assays |
| E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500 Tests × 20 |
