Mitochondrial Membrane Potential Assay Kit (with JC-1)

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中文名称：线粒体膜电位检测试剂盒(JC-1)

货号：E-CK-A301
文献（66）
说明书

说明书

Mitochondrial Membrane Potential Assay Kit (with JC-1)

Price: ¥800.00 ¥500.00 ¥260.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-JC-1

检测原理	线粒体膜电位下降是细胞凋亡早期的标志性事件，发生在细胞核凋亡特征（染色质浓缩、DNA断裂）出现之前，一旦线粒体膜电位崩溃，细胞凋亡便不可逆转。 JC-1是一种广泛用于检测线粒体膜电位 Ψm的理想荧光探针，可以检测细胞、组织或纯化的线粒体膜电位。JC-1有单体和多聚体两种存在形式，两者发射光谱不同。在正常细胞内，线粒体膜电位较高，JC-1聚集在线粒体的基质中，形成聚合物，可产生红色荧光；凋亡早期，线粒体膜电位降低，JC-1不能聚集在线粒体基质中，此时JC-1为单体，可产生绿色荧光。通过JC-1从红色荧光到绿色荧光的转变可检测到细胞膜电位的下降，可将JC-1荧光颜色的转变作为细胞凋亡早期的检测指标。常用红绿荧光的相对比例来衡量线粒体去极化的比例。 JC-1单体的最大激发波长为514 nm，最大发射波长为529 nm；JC-1聚合物的最大激发波长为585 nm，最大发射波长为590 nm。实际观察时，常规设置红色荧光和绿色荧光即可。
检测方法	荧光法;流式
样本类型	细胞
检测时间	40min
检测仪器	流式细胞仪;荧光显微镜
荧光	JC-1
Ex/Em	514/529, 585/590
Channel set	FITC, PE
自备试剂	超纯水
保存温度	该产品可在2~8°C/-20°C避光条件下保存12个月，具体试剂组分的保存条件请参考说明书。
有效期	12个月
运输条件	冰袋

本试剂盒检测喜树碱诱导的Jurkat细胞凋亡效果如图所示：正常细胞（左）存在少量凋亡，表现为出现少量线粒体膜电位崩塌细胞；诱导凋亡细胞（中, 2.5 μM喜树碱处理Jurkat细胞24 h）出现大量线粒体膜电位崩塌细胞；CCCP处理细胞（右，阳性对照）几乎所有细胞线粒体膜电位崩塌。

本试剂盒检测Hela细胞的线粒体膜电位变化：control（上），表现为极少量凋亡细胞；10 μM camptothecin（下）处理Hela细胞24 h，出现大量线粒体膜电位崩塌的凋亡细胞。

本试剂盒检测Hela细胞的线粒体膜电位变化：control（上），表现为少量线粒体膜电位崩塌细胞；10 μM CCCP（下）处理Hela细胞40 min，出现大量线粒体膜电位崩塌细胞。

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