Mitochondrial Membrane Potential Assay Kit (with JC-1)
中文名称：线粒体膜电位检测试剂盒(JC-1)
Price:
- 应用： 细胞凋亡-JC-1
| 检测原理 | 线粒体膜电位下降是细胞凋亡早期的标志性事件，发生在细胞核凋亡特征（染色质浓缩、DNA断裂）出现之前，一旦线粒体膜电位崩溃，细胞凋亡便不可逆转。
JC-1是一种广泛用于检测线粒体膜电位 Ψm的理想荧光探针，可以检测细胞、组织或纯化的线粒体膜电位。JC-1有单体和多聚体两种存在形式，两者发射光谱不同。在正常细胞内，线粒体膜电位较高，JC-1聚集在线粒体的基质中，形成聚合物，可产生红色荧光；凋亡早期，线粒体膜电位降低，JC-1不能聚集在线粒体基质中，此时JC-1为单体，可产生绿色荧光。
通过JC-1从红色荧光到绿色荧光的转变可检测到细胞膜电位的下降，可将JC-1荧光颜色的转变作为细胞凋亡早期的检测指标。常用红绿荧光的相对比例来衡量线粒体去极化的比例。
JC-1单体的最大激发波长为514 nm，最大发射波长为529 nm；JC-1聚合物的最大激发波长为585 nm，最大发射波长为590 nm。实际观察时，常规设置红色荧光和绿色荧光即可。 |
| 检测方法 | 荧光法;流式 |
| 样本类型 | 细胞 |
| 检测时间 | 40min |
| 检测仪器 | 流式细胞仪;荧光显微镜 |
| 荧光 | JC-1 |
| Ex/Em | 514/529, 585/590 |
| Channel set | FITC, PE |
| 自备试剂 | 超纯水 |
| 保存温度 | 该产品可在2~8°C/-20°C避光条件下保存12个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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