NFκB-p65 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

NFκB-p65 Polyclonal Antibody

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货号：E-AB-32233
文献（19）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC-p

Verified Samples	Verified Samples in WB: KB, Hela, 293T, 3T3 Verified Samples in IHC: Human stomach cancer, Mouse kidney
Dilution	WB 1:500-1:2000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from human NFκB-p65 around the non-phosphorylation site of Ser281.
Abbre	NFκB-p65
Synonyms	Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear fact, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3
Swissprot	Q04206
Calculated MW	60 kDa
Observed MW	60 kDa
Cellular Localization	Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Neuroscience, Signal Transduction
Conjugation	Unconjugated
Background	Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

Western Blot analysis of KB, Hela, 293T, 3T3 cells using NFκB-p65 Polyclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Human stomach cancer tissue using NFκB-p65 Polyclonal Antibody at dilution of 1:200.

Immunohistochemistry of paraffin-embedded Mouse kidney tissue using NFκB-p65 Polyclonal Antibody at dilution of 1:200.

pH-Responsive CuS/DSF/EL/PVP Nanoplatform Alleviates Inflammatory Bowel Disease in Mice via Regulating Gut Immunity and Microbiota

IF: 9.700

Journal： Acta Biomaterialia (2024)

DOI：10.1016/j.actbio.2024.02.034
Prevention of dextran sulfate sodium-induced acute enteritis by zinc nanoparticles green-formulated by sodium lignosulfonate

IF: 4.400

Journal： INORGANIC CHEMISTRY COMMUNICATIONS (2024)

DOI：10.1016/j.inoche.2024.112844

Reactivity：Rat
Mitigative role of cysteamine against unilateral renal reperfusion injury in Wistar rats

IF: 4.400

Journal： Frontiers in Pharmacology (2024)

DOI：10.3389/fphar.2024.1456903

Reactivity：Rat

Sample Type：renal tissue
Kaempferol sophoroside glucoside mitigates acetaminophen-induced hepatotoxicity: Role of Nrf2/NF-κB and JNK/ASK-1 signaling pathways

IF: 3.400

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e31448

Reactivity：Mouse

Sample Type：liver
Activation of angiotensin-converting enzyme 2 promotes hippocampal neurogenesis via activation of Wnt/β-catenin signaling in hypertension

IF: 2.600

Journal： MOLECULAR AND CELLULAR NEUROSCIENCE (2024)

DOI：10.1016/j.mcn.2024.103953

Reactivity：Rat

Sample Type：brain hippocampus tissue
Targeting Nrf2 with 3?H-1,2-dithiole-3-thione to moderate OXPHOS-driven oxidative stress attenuates IL-17A-induced psoriasis

IF: 7.500

Journal： BIOMEDICINE & PHARMACOTHERAPY (2023)

DOI：10.1016/j.biopha.2023.114294

PMID：36706632

Reactivity：Human
Anti-Inflammatory and Anti-Oxidative Effects of Polysaccharides Extracted from Unripe Carica papaya L. Fruit

IF: 7.000

Journal： Antioxidants (2023)

DOI：10.3390/antiox12081506

PMID：37627501

Reactivity：Human
Alginate/chitosan/diallyl disulfide nanoparticles: Synthesis, characterization and their anti-inflammatory efficacy on TPA-induced acute mouse ear inflammation

IF: 5.500

Journal： Carbohydrate Polymer Technologies and Applications (2023)

DOI：10.1016/j.carpta.2023.100362

Reactivity：Mouse

Sample Type：ears
Evaluating the Role of N-Acetyl-L-Tryptophan in the Aβ 1-42-Induced Neuroinflammation and Cognitive Decline in Alzheimer’s Disease

IF: 5.100

Journal： MOLECULAR NEUROBIOLOGY (2023)

DOI：10.1007/s12035-023-03844-4

PMID：38091207

Reactivity：Rat

Sample Type：hippocampus tissue,cortex tissue
The effect of Alnus incana (L.) Moench extracts in ameliorating iron overload-induced hepatotoxicity in male albino rats

IF: 4.600

Journal： Scientific Reports (2023)

DOI：10.1038/s41598-023-34480-6

PMID：37169909

Reactivity：Rat

Sample Type：Liver

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays