NFκB-p65 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

NFκB-p65 Polyclonal Antibody

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货号：E-AB-32233
文献（19）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC-p

Verified Samples	Verified Samples in WB: KB, Hela, 293T, 3T3 Verified Samples in IHC: Human stomach cancer, Mouse kidney
Dilution	WB 1:500-1:2000, IHC 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from human NFκB-p65 around the non-phosphorylation site of Ser281.
Abbre	NFκB-p65
Synonyms	Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear fact, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3
Swissprot	Q04206
Calculated MW	60 kDa
Observed MW	60 kDa
Cellular Localization	Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Neuroscience, Signal Transduction
Conjugation	Unconjugated
Background	Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

Western Blot analysis of KB, Hela, 293T, 3T3 cells using NFκB-p65 Polyclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Human stomach cancer tissue using NFκB-p65 Polyclonal Antibody at dilution of 1:200.

Immunohistochemistry of paraffin-embedded Mouse kidney tissue using NFκB-p65 Polyclonal Antibody at dilution of 1:200.

SIRT1/Nrf2/NF-κB Signaling Mediates Anti-Inflammatory and Anti-Apoptotic Activities of Oleanolic Acid in a Mouse Model of Acute Hepatorenal Damage

IF: 2.600

Journal： Medicina-Lithuania (2023)

DOI：10.3390/medicina59071351

PMID：37512162

Reactivity：Mouse

Sample Type：liver tissue,kidney tissue
Dopamine-modified chitosan hydrogel for spinal cord injury

IF: 10.723

Journal： CARBOHYDRATE POLYMERS (2022)

DOI：10.1016/j.carbpol.2022.120047

PMID：36241313

Reactivity：Mouse
Avanafil mediated dual inhibition of IKKβ and TNFR1 in an experimental paradigm of Alzheimer’s disease: in silico and in?vivo approach

IF: 5.235

Journal： JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2022)

DOI：10.1080/07391102.2022.2156924

PMID：36533331

Reactivity：Mouse

Sample Type：Brain
Microglial activation and neurobiological alterations in experimental autoimmune prostatitis-induced depressive-like behavior in mice

IF: 2.989

Journal： Neuropsychiatric Disease and Treatment (2022)

DOI：10.2147/NDT.S211288

PMID：31496706

Reactivity：Mouse

Sample Type：Serum,Tissue homogenate
LncRNA MALAT1 Facilitates Ovarian Cancer Progression through Promoting Chemoresistance and Invasiveness in the Tumor Microenvironment

IF: 5.924

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

DOI：10.3390/ijms221910201

PMID：34638541

Reactivity：Human
Lenvatinib Induces AKT/NF-κB Inactivation, Apoptosis Signal Transduction and Growth Inhibition of Non-small Cell Lung Cancer In Vivo

IF: 2.480

Journal： ANTICANCER RESEARCH (2021)

DOI：10.21873/anticanres.15068

Reactivity：Mouse

Sample Type：CL1-5-F4/NF-κB-luc2 cell-Xenograft
Protective effects of melatonin on the white matter damage of neonatal rats by regulating NLRP3 inflammasome activity

IF: 1.837

Journal： NEUROREPORT (2021)

DOI：10.1097/WNR.0000000000001642

PMID：33994520

Reactivity：Rat

Sample Type：periventricular
Chemopreventive effect of hesperidin, a citrus bioflavonoid in two stage skin carcinogenesis in Swiss albino mice

Journal： Heliyon (2019)

DOI：10.1016/j.heliyon.2019.e02521

PMID：31720442

Reactivity：Mouse

Sample Type：Tissue homogenate
Silibinin-induced autophagy mediated by PPARα-sirt1-AMPK pathway participated in the regulation of type I collagen-enhanced migration in murine 3T3-L1 preadipocytes

IF: 2.561

Journal： MOLECULAR AND CELLULAR BIOCHEMISTRY (2018)

DOI：10.1007/s11010-018-3368-y

PMID：29916120

Reactivity：Mouse

Sample Type：Cell lysate

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays