P53 Monoclonal Antibody
Price:
Size:
120 μL
60 μL
20 μL
- 宿主： Mouse
- 反应性： Human
- 应用性： WB;IHC-p;IF
| Verified Samples |
Verified Samples in WB: 293T Verified Samples in IHC: Human colon cancer, Verified Samples in IF: Hela |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:200 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide |
| Abbre | p53 |
| Synonyms | Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, P53, Phosphoprotein p53, TRP53, Tp53, Transformation related protein 53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53, p53, p53 tumor suppressor |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW | 53 kDa |
| Cellular Localization | Cytoplasm, Cytoplasm, Nucleus, Nucleus>PML body, Endoplasmic reticulum, Interaction with BANP promotes nuclear localization, Recruited into PML bodies together with CHEK2, Nucleus, Cytoplasm, Localized in both nucleus and cytoplasm in most cells, In some cells, forms foci in the nucleus that are different from nucleoli, Nucleus, Cytoplasm, Localized in the nucleus in most cells but found in the cytoplasm in some cells, Nucleus, Cytoplasm, Localized mainly in the nucleus with minor staining in the cytoplasm, Nucleus, Cytoplasm, Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus, Cytoplasm, Predominantly nuclear but translocates to the cytoplasm following cell stress. |
| Tissue Specificity | Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | 1H4 |
| Conjugation | Unconjugated |
| Background | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. |
Western Blot analysis of 293T cells using p53 Monoclonal Antibody at dilution of 1) 1:1000 2) 1:2000.
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using p53 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Hela tissue using p53 Monoclonal Antibody at dilution of 1:100.
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IGFBP7 is a key component of the senescence-associated secretory phenotype (SASP) that induces senescence in healthy cells by modulating the insulin, IGF, and activin A pathways
IF: 8.200 -
Targeting POLD1 to suppress the proliferation and migration of breast cancer MDA-MB-231 cell lines by downregulation of SIRT1
IF: 2.200 -
Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells
IF: 5.600 -
Beta-Tocotrienol Exhibits More Cytotoxic Effects than Gamma-Tocotrienol on Breast Cancer Cells by Promoting Apoptosis via a P53-Independent PI3-Kinase Dependent Pathway
IF: 4.082 -
Activation of p53 Gene Expression and Synergistic Antiproliferative Effects of 5-Fluorouracil and β-escin on MCF7 Cells
