PCNA Polyclonal Antibody
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Price:
Size:
100 μL
25 μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB: HepG2, Hela, RAW264.7, Raji, MCF-7 Verified Samples in IHC: Human colon cancer, Mouse testis, Rat testis |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Human Proliferating cell nuclear antigen protein |
| Abbre | PCNA |
| Synonyms | Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, PCNAR, Pcna/cyclin, Polymerase delta accessory protein, cb16, etID36690.10, fa28e03, fb36g03, ATLD2 |
| Swissprot | |
| Calculated MW | 28 kDa |
| Observed MW | 34 lDa |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell markers, Cancer, Isotype/Loading Controls |
| Conjugation | Unconjugated |
| Background | This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. |
Western Blot analysis of HepG2, Hela, Raw264.7 Raji and MCF-7 cells using PCNA Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human colon cancer using PCNA Polycloanl Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Mouse testis using PCNA Polycloanl Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Rat testis using PCNA Polycloanl Antibody at dilution of 1:50
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