PCNA Polyclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Rabbit
- 反应性： Human
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Jurkat, A431, 293T |
| Dilution | WB 1:3000-1:7000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide of Human Proliferating cell nuclear antigen |
| Abbre | PCNA |
| Synonyms | Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, PCNA, PCNAR, Pcna/cyclin, Polymerase delta accessory protein, Proliferating cell nuclear antigen, cb16, etID36690.10, fa28e03, fb36g03, ATLD2 |
| Swissprot | |
| Calculated MW | 28 kDa |
| Observed MW | 35 kDa |
| Cellular Localization | Nucleus |
| Tissue Specificity | Highest expressed in oocyte |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Background | Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. |
Western Blot analysis of Jurkat, A431 and 293T cells using PCNA Polyclonal Antibody at dilution of 1:6000.
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500 μL , 120 μL , 1 mL |
| E-IR-R304A | Western Blot Detection Kit | 50 Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50 Assays |
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