Recombinant Human IL-12 p40 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P29460
- 应用性： Cell culture
| 别称 |
IL-12/IL-23 p;IL-12 subunit p;NK cell stimulatory factor chain;NK cell Stimulating Factor Chain;CLMF p;IMD;IL12B;CLMF;CLMF2;IL-12B;IMD28;IMD29;NKSF;NKSF2;Cytotoxic lymphocyte maturation factor 40 kDa subunit;Cytotoxic Lymphocyte Maturation Factor 40 kDa subunit (CLMF p40);IL-12/IL-23 p40;NK cell Stimulating Factor Chain 2;p40;Interleukin-12 subunit beta;CLMF p40;IL-12 subunit p40;NK cell stimulatory factor chain 2;IL 12B;IL12 p40;Interleukin 12 beta chain;Interleukin 12 p40
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| 表达系统 |
E.coli
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| 序列 |
Ile 23-Ser 328
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| 蛋白编码 |
P29460
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| 种属 |
Human
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| 计算分子量 |
35.6 kDa
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| 表观分子量 |
45 kDa
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| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to induce cell proliferation in PHA-activated human peripheral blood lymphocytes (PBMC) using a concentration range of 5-50 ng/mL. Note: Results may vary from different PBMC donors.
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| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-12 subunit beta (IL-12B) belongs to the type I cytokine receptor family. It contains 1 fibronectin type-III domain and 1 Ig-like C2-type domain. IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor encoded by IL12B and a 35 kD subunit encoded by IL12A. IL12 is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. It has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.
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实验操作视频
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