Recombinant Mouse Agrp Protein(GST Tag)
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- 表达系统： E.coli
- 蛋白编码： P56473
| 别称 |
ASIP;ASIP2;Agrt;Art;Agrp
|
| 表达系统 |
E.coli
|
| 序列 |
Val21-Thr131
|
| 蛋白编码 |
P56473
|
| 种属 |
Mouse
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| 计算分子量 |
38.1 kDa
|
| 表观分子量 |
38 kDa
|
| 标签 |
N-GST
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 85% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
|
| 背景 |
Agouti Related Protein (AGRP, or AGRT), is an endogenous antagonist of the melanocortin receptors MC3R and MC4R found in the hypothalamus and exhibits potent orexigenic activity. AGRP can act as a competitive antagonist to proopiomelanocortin (POMC)-derived peptides at the melanocortin-4 receptor (MC4R), and that this homeostatic mechanism is important as a means of coordinating appetite with perceived metabolic requirement. AGRP is upregulated by fasting while intracerebroventricular injections of synthetic AGRP lead to increased appetite and food intake. Thus, AGRP is a powerful orexigenic peptide that increases food intake when ubiquitously overexpressed or when administered centrally.
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实验操作视频
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