SMAD3 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

SMAD3 Polyclonal Antibody

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货号：E-AB-13066
文献（3）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC

Verified Samples	Verified Samples in WB: Human kidney Verified Samples in IHC: Mouse colon, Human thyroid cancer
Dilution	WB 1:500-1:2000, IHC 1:25-1:100
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthetic peptide of human SMAD3
Abbre	SMAD3
Synonyms	DKFZP586N0721, DKFZp686J10186, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, hMAD 3, hMAD-3, hSMAD3, mothers against decapentaplegic homolog 3
Swissprot	P84022
Calculated MW	48 kDa
Cellular Localization	Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).
Concentration	0.3 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% sodium azide and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Stem Cells
Conjugation	Unconjugated
Background	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Western Blot analysis of Human kindey cancer tissue using SMAD3 Polyclonal Antibody at dilution of 1:400

Immunohistochemistry of paraffin-embedded Mouse colon using SMAD3 Polyclonal Antibody at dilution of 1:50

Immunohistochemistry of paraffin-embedded Human thyroid cancer using SMAD3 Polyclonal Antibody at dilution of 1:25

Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice

IF: 14.260

Journal： EMBO Molecular Medicine (2022)

DOI：10.15252/emmm.202216244

Reactivity：Mouse

Sample Type：Tibialis anterior skeletal muscles ,colonic tissues
Diesel exhaust exposure in mice induces pulmonary fibrosis by TGF-β/Smad3 signaling pathway

IF: 7.963

Journal： SCIENCE OF THE TOTAL ENVIRONMENT (2021)

DOI：10.1016/j.scitotenv.2021.150623

PMID：34610407

Reactivity：Mouse

Sample Type：Lung
Protective effect of trimetazidine in radiation-induced cardiac fibrosis in mice

IF: 1.950

Journal： JOURNAL OF RADIATION RESEARCH (2020)

DOI：10.1093/jrr/rraa043

PMID：32642776

Reactivity：Mouse

Sample Type：heart

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays