Streptavidin Polyclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Rabbit
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: 10ng Streptavidin (16kDa) |
| Dilution | WB 1:500-2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Native Streptomyces Avidinii streptavidin protein |
| Abbre | Streptavidin |
| Synonyms | Avidin, SA, Streptavidin |
| Swissprot | |
| Calculated MW | 40 kDa |
| Observed MW | 40 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces. Streptavidin can bind to four moles of biotin per mole of protein with extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does. |
Western blotting of Streptavidin with anti-Streptavidin rabbit polyclonal antibody at dilution of 1:1000. Lane 1 : Recombinant Streptavidin protein at 6.25ng; Lane 2 : Recombinant Streptavidin protein at 12.5ng; Lane 3: Recombinant Streptavidin protein at 25ng; Lane 4: Recombinant Streptavidin protein at 50ng
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500 μL , 120 μL , 1 mL |
| E-IR-R304A | Western Blot Detection Kit | 50 Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50 Assays |
