Annexin V-FITC/PI Apoptosis Kit
中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒(即用型Buffer)

Price:
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光法;流式 |
样本类型 | 细胞 |
检测时间 | 40 min |
检测仪器 | 流式细胞仪 |
荧光 | FITC |
Ex/Em | 490/530 |
Channel set | FITC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
运输条件 | 冰袋 |
-
Activating the cGAS-STING Pathway by Manganese-Based Nanoparticles Combined with Platinum-Based Nanoparticles for Enhanced Ovarian Cancer Immunotherapy
IF: 15.800Journal: ACS Nano (2025) -
A Dynamic Deferoxamine Polymer with Exceptional Performance in Mitochondrial Iron Depletion and Cytosolic Protein Delivery
IF: 13.000Journal: Small (2025) -
BRD4-targeted photodegradation nanoplatform for light activatable melanoma therapy
IF: 12.800Journal: BIOMATERIALS (2025) -
Biomimetic Nanoplatform-Mediated Protective Autophagy Blockage Enhancing Sonodynamic and Ca2+-Overload Combined Therapy for Colon Cancer
IF: 10.700Journal: Small Methods (2025) -
Multifunctional porous organic polymer-based hybrid nanoparticles for sonodynamically enhanced cuproptosis and synergistic tumor therapy
IF: 9.400Journal: Acta Biomaterialia (2025) -
EBF1-induced CSRP2 boosts the progression of B-cell acute lymphocytic leukemia by inhibiting ferroptosis
IF: 9.100Journal: CANCER LETTERS (2025) -
Curcumin-loaded bioadhesive silk fibroin microsphere improves islet transplantation by mitigating oxidative stress and inhibiting apoptosis
IF: 8.700Journal: Materials Today Bio (2025) -
Multifunctional hydrogel loaded with 4-octyl itaconate and exosomes to induce bone regeneration for diabetic infected bone defect via Keap1-Nrf2 pathway
IF: 8.700Journal: Materials Today Bio (2025) -
Shenghui Decoction inhibits neuronal cell apoptosis to improve Alzheimer's disease through the PDE4B/cAMP/CREB signaling pathway
IF: 6.700Journal: PHYTOMEDICINE (2025) -
Palmatine activation of TFEB enhances autophagy and alleviates endoplasmic reticulum stress in intervertebral disc degeneration
IF: 6.700Journal: PHYTOMEDICINE (2025)
E-CK-A111 | Annexin V-FITC Reagent | 500 Tests , 200 Tests , 100 Tests , 50 Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50 mL , 10 mL |
E-CK-A212 | Annexin V-FITC/7-AAD Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A252 | Annexin V-FITC/DAPI Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100 Assays , 50 Assays , 20 Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20 Assays |