Annexin V-FITC/PI Apoptosis Kit
中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒(即用型Buffer)

Price:
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光法;流式 |
样本类型 | 细胞 |
检测时间 | 40 min |
检测仪器 | 流式细胞仪 |
荧光 | FITC |
Ex/Em | 490/530 |
Channel set | FITC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
运输条件 | 冰袋 |
-
Curcumin-loaded bioadhesive silk fibroin microsphere improves islet transplantation by mitigating oxidative stress and inhibiting apoptosis
IF: 8.7Journal: Materials Today Bio (2025) -
Multifunctional hydrogel loaded with 4-octyl itaconate and exosomes to induce bone regeneration for diabetic infected bone defect via Keap1-Nrf2 pathway
IF: 8.7Journal: Materials Today Bio (2025) -
Shenghui Decoction inhibits neuronal cell apoptosis to improve Alzheimer's disease through the PDE4B/cAMP/CREB signaling pathway
IF: 6.7Journal: PHYTOMEDICINE (2025) -
Palmatine activation of TFEB enhances autophagy and alleviates endoplasmic reticulum stress in intervertebral disc degeneration
IF: 6.7Journal: PHYTOMEDICINE (2025) -
COPB1 deficiency triggers osteoporosis with elevated iron stores by inducing osteoblast ferroptosis
IF: 5.9Journal: Journal of Orthopaedic Translation (2025) -
Enzymatic Synthesis of Biflavonoid Glycosides with Enhanced Antitumor Activity Using Glycosyltransferase and Sucrose Synthase
IF: 5.7Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2025) -
MSC-mediated mitochondrial transfer promotes metabolic reprograming in endothelial cells and vascular regeneration in ARDS
IF: 5.2Journal: REDOX REPORT (2025) -
Investigation of Anti-Cancer Properties of Novel Curcuminoids in Leukemic Cells and Dalton Lymphoma Ascites Model
IF: 4.9Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025) -
Maackiain induces apoptosis and autophagy via ROS-mediated endoplasmic reticulum stress in endometrial cancer
IF: 4.8Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2025) -
Protective effect of A-type ECG dimer on oxidative damage of retinal pigment epithelial cell
IF: 4.8Journal: Food Bioscience (2025)
E-CK-A111 | Annexin V-FITC Reagent | 500 Tests , 200 Tests , 100 Tests , 50 Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50 mL , 10 mL |
E-CK-A212 | Annexin V-FITC/7-AAD Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A252 | Annexin V-FITC/DAPI Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100 Assays , 50 Assays , 20 Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20 Assays |
Q11:Annexin V试剂盒可以检测植物样品吗?
我司没有进行过植物样本的验证,如果您有需的话,可以做预实验看下效果。
Q12:Annexin V的实验温度选择4℃可以吗?
建议室温孵育,25℃左右。Annexin V实验的染色过程实际上是使AnnexinV蛋白与细胞膜上的磷脂酰丝氨酸特异结合的过程,这个过程在细胞功能和酶活性正常的状态下进行为宜。
Q13:Annexin V可以做小鼠海马组织细胞凋亡吗?
不建议老师使用组织样本做Annexin V实验。一方面,组织样本制备成单细胞悬液的操作过程中,实验操作导致的凋亡可能比样本本身的凋亡率高,而且实验操作具有不稳定性,最终的实验无法判断出样本本身的实际凋亡率。另一方面,组织样本存在多种细胞类型,每种细胞的自发荧光不同,结果无法分析。
Q14:Annexin V凋亡检测试剂盒中一步法和两步法的区别?
一步法操作更简单,两步法由于减小了孵育体系,所以染料用量减半,更节省试剂,从结果上来看,一步法与两步法的结果分辨率一致。