Annexin V-FITC/PI Apoptosis Kit

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中文名称：Annexin V-FITC / PI细胞凋亡检测试剂盒（即用型Buffer）

货号：E-CK-A211B
文献（70）
说明书

说明书

Price: ¥2400.00 ¥1500.00 ¥900.00 ¥450.00

Size:

200 Assays 100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Annexin V

检测原理	Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。
检测方法	荧光法;流式
样本类型	细胞
检测时间	40 min
检测仪器	流式细胞仪
荧光	FITC
Ex/Em	490/530
Channel set	FITC
自备试剂	PBS(E-BC-R187), 去离子水
保存温度	该产品可在 2~8°C避光条件下保存6个月。
有效期	6个月
运输条件	冰袋

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