Pan Acetyl-Lysine Polyclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Rabbit
- 反应性: All
- 应用性: WB;Dot blot
Verified Samples |
Verified Samples in Dot blot: BSA Verified Samples in WB: E.coli |
Dilution | WB 1:1000-2000, Dot Blot 1:2000 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Acetylated Protein |
Abbre | Pan Acetyl-Lysine |
Synonyms | Anti-acetyl Lysine, pan acetyl Lysine antibody |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Antigen Affinity Purification |
Conjugation | Unconjugated |
Background | Acetylation is an important modification of proteins in cell biology. Acetylation occurs in thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport. In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription.The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis. |
Dot blotting analysis of Pan Acetyl-Lysine polyclonal antibody on unmodified BSA and acetylated BSA at dilution of 1:2000.
Western blotting of anti-Pan Acetyl-Lysine polyclonal antibody on E.coli lysates at dilution of 1:500.
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