AMPK alpha2 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

AMPK alpha2 Polyclonal Antibody

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货号：D-AB-10161L
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说明书

说明书

Price: ¥2100.00 ¥800.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB: Human Hep G2, Human Hela, Mouse NIH/3T3 Verified Samples in IHC: Human breast Verified Samples in IF: Human MCF7
Dilution	WB 1:500-1:1000, IHC 1:100-1:200, IF 1:100-1:400
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Rat Ampk2 protein expressed by E.coli
Abbre	AMPK
Synonyms	5'-AMP-activated protein kinase catalytic subunit alpha-2, AAPK2, ACACA kinase, AMPK alpha 2 chain, AMPK subunit alpha-2, AMPK2, AMPKa2, AMPKalpha2, Acetyl-CoA carboxylase kinase, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA, PRKAA2, Protein kinase
Swissprot	Q09137
Calculated MW	62 kDa
Observed MW	62 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cancer, Cardiovascular, Metabolism, Signal Transduction
Conjugation	Unconjugated
Background	The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Western blot with Ampk2 Polyclonal antibody at dilution of 1:1000.lane 1:Hep G2 whole cell lysate, lane 2:Hela whole cell lysate, lane 3:NIH/3T3 whole cell lysate

Immunohistochemistry of paraffin-embedded Human breast using AMPK alpha2 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of McF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of MCF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100

Inhibition of lactate dehydrogenase A by diclofenac sodium induces apoptosis in HeLa cells through activation of AMPK

IF: 5.500

Journal： FEBS Journal (2024)

DOI：10.1111/febs.17158

Reactivity：Human

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays