Annexin V-APC/PI Apoptosis Kit
中文名称:Annexin V-APC / PI细胞凋亡检测试剂盒

Price:
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光法;流式 |
样本类型 | 细胞 |
检测时间 | 40 min |
检测仪器 | 流式细胞仪 |
荧光 | APC |
Ex/Em | 650/660 |
Channel set | APC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
-
Polymer-based nanodrugs enhance sonodynamic therapy through epigenetic reprogramming of the immunosuppressive tumor microenvironment
IF: 10.500Journal: JOURNAL OF CONTROLLED RELEASE (2025) -
Ubiquitin-conjugating enzyme E2S decreases the sensitivity of glioblastoma cells to temozolomide by upregulating PGAM1 via the interaction with OTUB2
IF: 7.700Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025) -
SRT3025-loaded cell membrane hybrid liposomes (3025@ML) enhanced anti-tumor activity of Oxaliplatin via inhibiting pyruvate kinase M2 and fatty acid synthase
IF: 3.900Journal: Lipids in Health and Disease (2025) -
Development of carbazole-based molecules for inhibition of mutant hSOD1 protein aggregation in Amyotrophic Lateral Sclerosis
IF: 3.300Journal: BIOORGANIC & MEDICINAL CHEMISTRY (2025) -
miR-124-3p inhibits CRC proliferation, migration, and invasion by targeting ITGB1
IF: 2.800Journal: Discover Oncology (2025) -
Combination of oxabicycloheptene sulfonate (OBHS) and antidiabetic drugs synergistically inhibits proliferation in human breast cancer cells
IF: 1.400Journal: Oncologie (2025) -
Stem Leydig cells support macrophage immunological homeostasis through mitochondrial transfer in mice
IF: 16.600 -
SNAI1 promotes epithelial-mesenchymal transition and maintains cancer stem cell-like properties in thymic epithelial tumors through the PIK3R2/p-EphA2 Axis
IF: 11.400Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2024) -
Ubiquitin-independent degradation of Bim blocks macrophage pyroptosis in sepsis-related tissue injury
IF: 8.100Journal: Cell Death & Disease (2024) -
Conductive, injectable hydrogel equipped with tetramethylpyrazine regulates ferritinophagy and promotes spinal cord injury repair
IF: 7.700Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)
E-CK-A117 | Annexin V-APC Reagent | 500 Tests , 200 Tests , 100 Tests , 50 Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50 mL , 10 mL |
E-CK-A218 | Annexin V-APC/7-AAD Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A258 | Annexin V-APC/DAPI Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100 Assays , 50 Assays , 20 Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20 Assays |