Annexin V-FITC/PI Apoptosis Kit
中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒

Price:
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光法;流式 |
样本类型 | 细胞 |
检测时间 | 40 min |
检测仪器 | 流式细胞仪 |
荧光 | FITC |
Ex/Em | 490/530 |
Channel set | FITC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
-
Integrated metabolomics and proteomics revealed mangiferin alleviating oxidative stress and mitochondrial dysfunction of HT22?cells via regulating the abnormal metabolic pathways
IF: 4.800Journal: Food Bioscience (2025) -
Placental trophoblast aging in advanced maternal age is related to increased oxidative damage and decreased YAP
IF: 4.600Journal: Frontiers in Cell and Developmental Biology (2025) -
Adenine Nucleotide Translocase 1 Promotes Functional Integrity of Mitochondria via Activating DDIT3-CytC Pathway and Intensifying Actin Filament Structures
IF: 4.600Journal: MOLECULAR NEUROBIOLOGY (2025) -
Sirtuin1 Deficiency Could Exacerbate Melanocyte Apoptosis Under Endoplasmic Reticulum Stress
IF: 4.500Journal: INFLAMMATION (2025) -
Decrease of NAD+ Inhibits the Apoptosis of OLP T Cells via Inducing Mitochondrial Fission
IF: 4.200Journal: Journal of Inflammation Research (2025) -
Serum Starvation Enhances the Antitumor Activity of Natural Matrices: Insights into Bioactive Molecules from Dromedary Urine Extracts
IF: 4.200Journal: MOLECULES (2025) -
Tilia species (linden) exert anti-cancer effects on MIA PaCa-2 cells through the modulation of oxidative stress and inflammation
IF: 3.800Journal: Scientific Reports (2025) -
Investigation of Scutellaria Barbata’s immunological mechanism against thyroid cancer using network pharmacology and experimental validation
IF: 3.800Journal: Scientific Reports (2025) -
In vitro analysis of the molecular mechanisms of ursolic acid against ovarian cancer
IF: 3.300Journal: BMC Complementary Medicine and Therapies (2025) -
Sodium butyrate attenuates microglia-mediated neuroinflammation by modulating the TLR4/MyD88/NF-κB pathway and microbiome-gut-brain axis in cardiac arrest mice
IF: 3.300Journal: Molecular Brain (2025)
E-CK-A111 | Annexin V-FITC Reagent | 500 Tests , 200 Tests , 100 Tests , 50 Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50 mL , 10 mL |
E-CK-A212 | Annexin V-FITC/7-AAD Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A252 | Annexin V-FITC/DAPI Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100 Assays , 50 Assays , 20 Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20 Assays |