Annexin V-FITC/PI Apoptosis Kit
中文名称：Annexin V-FITC / PI细胞凋亡检测试剂盒
Price:
- 应用： 细胞凋亡-Annexin V
| 检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。
细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。 |
| 检测方法 | 荧光法;流式 |
| 样本类型 | 细胞 |
| 检测时间 | 40 min |
| 检测仪器 | 流式细胞仪 |
| 荧光 | FITC |
| Ex/Em | 490/530 |
| Channel set | FITC |
| 自备试剂 | PBS(E-BC-R187), 去离子水 |
| 保存温度 | 该产品可在 2~8°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
-
M13, an anthraquinone compound isolated from Morinda officinalis alleviates the progression of the osteoarthritis via the regulation of STAT3
IF: 6.700Journal： PHYTOMEDICINE (2024) -
STK3 kinase activation inhibits tumor proliferation through FOXO1-TP53INP1/P21 pathway in esophageal squamous cell carcinoma
IF: 6.600 -
RAPSYN-mediated neddylation of BCR-ABL alternatively determines the fate of Philadelphia chromosome-positive leukemia
IF: 6.400Journal： eLife (2024) -
Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas
IF: 6.100Journal： Journal of Translational Medicine (2024) -
S1PR3 inhibition protects against LPS-induced ARDS by inhibiting NF-κB and improving mitochondrial oxidative phosphorylation
IF: 6.100Journal： Journal of Translational Medicine (2024) -
FAT10 induces immune suppression by upregulating PD-L1 expression in hepatocellular carcinoma
IF: 6.100Journal： APOPTOSIS (2024) -
CD8?+?CD103?+?iTregs protect against ischemia-reperfusion-induced acute kidney Injury by inhibiting pyroptosis
IF: 6.100Journal： APOPTOSIS (2024) -
Vasorin (VASN) overexpression promotes pulmonary metastasis and resistance to adjuvant chemotherapy in patients with locally advanced rectal cancer
IF: 6.100Journal： Journal of Translational Medicine (2024) -
Transcriptional activation of PINK1 by MyoD1 mediates mitochondrial homeostasis to induce renal calcification in pediatric nephrolithiasis
IF: 6.100Journal： Cell Death Discovery (2024) -
N-acetyltransferase 10 affects the proliferation of intrahepatic cholangiocarcinoma and M2-type polarization of macrophages by regulating C-C motif chemokine ligand 2
IF: 6.100Journal： Journal of Translational Medicine (2024)
| E-CK-A111 | Annexin V-FITC Reagent | 500Tests , 200 Tests , 100 Tests , 50 Tests |
| E-CK-A151 | Annexin V Binding Buffer (10×) | 50mL , 10mL |
| E-CK-A212 | Annexin V-FITC/7-AAD Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A252 | Annexin V-FITC/DAPI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20Assays |
