Bcl-2 Polyclonal Antibody
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Price:
Size:
200 μL
120 μL
60 μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC;IF
| Verified Samples |
Verified Samples in WB: HL-60, HL-60, Mouse testis, Rat lung, Rat liver Verified Samples in IHC: Human lung cancer, Rat brain, Mouse testis Verified Samples in IF: C6, NIH/3T3, U-2OS |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100, IF 1:50-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human Bcl-2 |
| Abbre | Bcl-2 |
| Synonyms | BCL2, Bcl-2, PPP1R50 |
| Swissprot | |
| Calculated MW | 22 kDa/26 kDa |
| Observed MW | 24kDa/26 kDa |
| Cellular Localization | Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Background | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
Western blot analysis of extracts of HL-60 cells using Bcl-2 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of extracts of various cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human lung cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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