Bcl-2 Polyclonal Antibody（E-AB-60012）|-Elabscience伊莱瑞特【官网】

Bcl-2 Polyclonal Antibody

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货号：E-AB-60012
文献（51）
说明书

说明书

Price: ¥3580.00 ¥2220.00 ¥1380.00

Size:

200 μL 120 μL 60 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB: HL-60, HL-60, Mouse testis, Rat lung, Rat liver Verified Samples in IHC: Human lung cancer, Rat brain, Mouse testis Verified Samples in IF: C6, NIH/3T3, U-2OS
Dilution	WB 1:500-1:2000, IHC 1:50-1:100, IF 1:50-1:100
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide of human Bcl-2
Abbre	Bcl-2
Synonyms	BCL2, Bcl-2, PPP1R50
Swissprot	P10415
Calculated MW	22 kDa/26 kDa
Observed MW	24kDa/26 kDa
Cellular Localization	Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Purification Method	Affinity purification
Research Areas	Cancer, Cell Biology, Metabolism, Signal Transduction
Conjugation	Unconjugated
Background	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Western blot analysis of extracts of HL-60 cells using Bcl-2 Polyclonal Antibody at dilution of 1:500.

Western blot analysis of extracts of various cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded Human lung cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat brain using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Effect of baicalin and baicalin-bovine serum albumin nanoparticle against bendiocarb exposure in rats

IF: 2.2

Journal： Toxicology Research (2024)

DOI：10.1093/toxres/tfae134

Reactivity：Rat

Sample Type：kidney tissue,liver tissue
Effects of 630?nm laser on apoptosis, metastasis, invasion and epithelial-to-mesenchymal transition of human lung squamous cell carcinoma H520 cells mediated by hematoporphyrin derivatives

IF: 2.1

Journal： LASERS IN MEDICAL SCIENCE (2024)

DOI：10.1007/s10103-024-04176-y

Reactivity：Human
Hederagenin Induces Apoptosis of Human Hepatoma HepG2 Cells via the Mitochondrial Pathway

IF: 1.6

Journal： COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING (2024)

DOI：10.2174/0113862073254353230925074944

Reactivity：Human
lncRNA CCAT2 Protects Against Cardiomyocyte Injury After Myocardial Ischemia/Reperfusion by Regulating BMI1 Expression

IF: 1.5

Journal： International Heart Journal (2024)

DOI：10.1536/ihj.23-569

PMID：38556336

Reactivity：Human
Analysis of Co-expression of HSP 70 and BCL 2 in Oral Precancer and Cancer Patients Using Immunohistochemistry

IF: 0.8

Journal： Journal of Maxillofacial & Oral Surgery (2024)

DOI：10.1007/s12663-024-02193-6

Reactivity：Human

Sample Type：oral epithelium
Long-term consumption of fermented pork fat-based diets differing in calorie, fat content, and fatty acid levels mediates oxidative stress, inflammation, redox imbalance, germ cell apoptosis, disruption of steroidogenesis, and testicular dysfunction in Wistar rats

IF: 5.800

Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)

DOI：10.1007/s11356-023-26018-0

PMID：36840878

Reactivity：Rat

Sample Type：testis tissue
Hexarelin alleviates apoptosis on ischemic acute kidney injury via MDM2/p53 pathway

IF: 4.2

Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2023)

DOI：10.1186/s40001-023-01318-w

PMID：37710348

Reactivity：Rat，Human

Sample Type：kidney
Pleiotropic attenuating effect of Ginkgo biloba against isoprenaline-induced myocardial infarction via improving Bcl-2/mTOR/ERK1/2/Na+, K+-ATPase activities

IF: 3.8

Journal： Chinese Herbal Medicines (2023)

DOI：10.1016/j.chmed.2023.11.001

Reactivity：Rat

Sample Type：heart tissue
Ginkgo biloba supplement abates lead-induced endothelial and testicular dysfunction in Wistar rats via up-regulation of Bcl-2 protein expression, pituitary-testicular hormones and down-regulation of oxido-inflammatory reactions

IF: 3.500

Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2023)

DOI：10.1016/j.jtemb.2023.127216

PMID：37224746

Reactivity：Rat

Sample Type：aorta,testes
Ginkgo biloba modulates ET-I/NO signalling in Lead Acetate induced rat model of endothelial dysfunction: Involvement of oxido-inflammatory mediators

IF: 3.200

Journal： INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2023)

DOI：10.1080/09603123.2023.2194612

PMID：36960596

Reactivity：Rat

Sample Type：Aorta fixed-tissues

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